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Species: Synthetic
Genetic Insert: M.XhoI
Vector Backbone Description: Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35255734
Proper citation: RRID:Addgene_170768 Copy
Species: Arabidopsis thaliana
Genetic Insert: GID1-VPR-IRES-HygR
Vector Backbone Description: Vector Backbone:pSLQ2842 (Addgene #84244); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_170808 Copy
Vector Backbone Description: Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.
Proper citation: RRID:Addgene_170809 Copy
Species: Drosophila melanogaster
Genetic Insert: GFP-Msp300KASH
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid derived from GFP-Msp300KASH fusion provided by Janice Fischer and described in:
Yu, J. et al. (2006) The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental biology 289, 336-345, doi:10.1016/j.ydbio.2005.10.027
Plasmid first described in:
Ma, J. and Weake, V.M. (2014). Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Journal of Visualized Experiments 85. PMID:24686501.
Proper citation: RRID:Addgene_170806 Copy
Species: Drosophila melanogaster
Genetic Insert: 2xFLAG/mCherry-Msp300KASH
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid first described in:
Hall H., Medina P., Cooper D.A., Escobedo S.E., Rounds J., Brennan K.J., Vincent C., Miura P., Doerge R. and Weake V.M. (2017). Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence. BMC Genomics. 18(1):894. PMID: 29162050.
Proper citation: RRID:Addgene_170807 Copy
Species: Synthetic
Genetic Insert: APV-SBP-Dendra2-ER-5
Vector Backbone Description: Backbone Marker:Franck Perez Lab (Addgene #65286); Backbone Size:4407; Vector Backbone:Str-KDEL_SBP-EGFP-Ecadherin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35051356
Proper citation: RRID:Addgene_170679 Copy
Species: Synthetic
Genetic Insert: APV-SBP-DsRed2-ER-5
Vector Backbone Description: Backbone Marker:Franck Perez Lab (Addgene #65286); Backbone Size:4407; Vector Backbone:Str-KDEL_SBP-EGFP-Ecadherin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35051356
Comments: Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory.
Proper citation: RRID:Addgene_170675 Copy
Species: Synthetic
Genetic Insert: EPV-SBP-DsRed2-ER-5
Vector Backbone Description: Backbone Marker:Franck Perez Lab (Addgene #65286); Backbone Size:4407; Vector Backbone:Str-KDEL_SBP-EGFP-Ecadherin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35051356
Comments: Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory.
Proper citation: RRID:Addgene_170676 Copy
Species: firefly
Genetic Insert: luc
Vector Backbone Description: Backbone Marker:from Han lab; Backbone Size:8800; Vector Backbone:pBOBI_cs2.0; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32540903
Proper citation: RRID:Addgene_170674 Copy
Species: Homo sapiens
Genetic Insert: ERGIC-53
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4026; Vector Backbone:pDendra2-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051356
Comments: Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory.
Proper citation: RRID:Addgene_170717 Copy
Species: Synthetic
Genetic Insert: APV-DsRed2-mEmerald-ER-5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4026; Vector Backbone:pDendra2-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051356
Comments: Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory.
Proper citation: RRID:Addgene_170715 Copy
Genetic Insert: csgA
Vector Backbone Description: Backbone Size:1912; Vector Backbone:pZA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30848859
Proper citation: RRID:Addgene_170787 Copy
Species: Homo sapiens
Genetic Insert: STAT5A
Vector Backbone Description: Vector Backbone:pTRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34263728
Comments: A constitutively active form of STAT5A was cloned into the lentiviral expression vector.
Proper citation: RRID:Addgene_170662 Copy
Species: Synthetic
Genetic Insert: ZnGreen2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539450
Comments: When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments.
Proper citation: RRID:Addgene_170781 Copy
Species: Homo sapiens
Genetic Insert: W96A-GLTP
Vector Backbone Description: Vector Backbone:pET30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15329726
Proper citation: RRID:Addgene_170734 Copy
Species: Homo sapiens
Genetic Insert: GLTP
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30206120
Proper citation: RRID:Addgene_170732 Copy
Species: Homo sapiens
Genetic Insert: GLTP
Vector Backbone Description: Vector Backbone:pET30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15329726
Proper citation: RRID:Addgene_170733 Copy
Species: Arabidopsis thaliana
Genetic Insert: acd11
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24412362
Proper citation: RRID:Addgene_170738 Copy
Species: Danio rerio
Genetic Insert: Dapper-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. cloned into Cla1/Xba1 sites.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17082 Copy
Species: Homo sapiens
Genetic Insert: K60A-CPTP
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23863933
Proper citation: RRID:Addgene_170736 Copy
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