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Species: Synthetic
Genetic Insert: NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR
Vector Backbone Description: Backbone Marker:Caroline Goujon (Addgene plasmid # 139449); Vector Backbone:LentiGuide-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139454 Copy
Species: Synthetic
Genetic Insert: Non-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139455 Copy
Species: Synthetic
Genetic Insert: NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139458 Copy
Species: Synthetic
Genetic Insert: NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139459 Copy
Species: Synthetic
Genetic Insert: IFITM3-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139460 Copy
Species: Synthetic
Genetic Insert: Curlcake4 with T7 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501426
Proper citation: RRID:Addgene_139343 Copy
Species: Synthetic
Genetic Insert: Curlcake2 with T7 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501426
Proper citation: RRID:Addgene_139341 Copy
Species: Synthetic
Genetic Insert: Scarlet 10AA Neon green
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32191861
Comments: we used #98839 (mScarlet-I-mTurquoise2) as a base for the vector and replaced Turquoise2 with Neon green.
Proper citation: RRID:Addgene_139364 Copy
Species: Synthetic
Genetic Insert: Turquoise1 78AA Venus
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Turquoise -5-Venus was the base plasmid and a 78 AA linker was added.
This is a low FRET efficiency standard.
Jameson, D.M. (2016) Perspectives on Fluorescence : a Tribute to Gregorio Weber. In Springer Series on Fluorescence, Methods and Applications, , pp. 143-161.
Proper citation: RRID:Addgene_139372 Copy
Species: Synthetic
Genetic Insert: d4Venus
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139552 Copy
Species: Synthetic
Genetic Insert: DsRedT4
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139550 Copy
Species: Synthetic
Genetic Insert: rtTA3G
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139556 Copy
Species: Synthetic
Genetic Insert: mKO1
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139553 Copy
Species: Synthetic
Genetic Insert: Hygromycin resistant gene
Vector Backbone Description: Backbone Size:2582; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139559 Copy
Species: Synthetic
Genetic Insert: PGK-BSD-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:12702; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_139562 Copy
Species: Synthetic
Genetic Insert: PGK-ZeoR-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:12956; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_139563 Copy
Species: Synthetic
Genetic Insert: MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33164485
Comments: Mutation V554L has no effect on the function of HO1.
Proper citation: RRID:Addgene_139479 Copy
Species: Synthetic
Genetic Insert: CAG rox stop rox td-sfGFP dre reporter
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-4; Vector Types:Mammalian Expression, Mouse Targeting, Dre/rox; Bacterial Resistance:Ampicillin
Comments: Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive G-C region. Depositor confirms these bases do not affect plasmid function.
Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
In plasmids constructed later for this collection, the four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE. For example, see https://www.addgene.org/177150/.
Proper citation: RRID:Addgene_139513 Copy
Species: Synthetic
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
Vector Backbone Description: Backbone Size:5208; Vector Backbone:pT2Apuro-TRE; Vector Types:Mammalian Expression, Tol2 transposon donor vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33164485
Proper citation: RRID:Addgene_139481 Copy
Species: Synthetic
Genetic Insert: rtTA, Tet-ON reverse transactivator
Vector Backbone Description: Backbone Size:10830; Vector Backbone:pCSIIhyg; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33164485
Proper citation: RRID:Addgene_139480 Copy
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