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Species: Mus musculus
Genetic Insert: MOR283-5
Vector Backbone Description: Backbone Marker:promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17350283
Proper citation: RRID:Addgene_15851 Copy
Species: Synthetic
Genetic Insert: PE2
Vector Backbone Description: Vector Backbone:pB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_158567 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_158565 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_158566 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: 5' Cloning Site: PacI (not destroyed), 3' Cloning Site: SbfI (not destroyed)
Proper citation: RRID:Addgene_158563 Copy
Vector Backbone Description: Backbone Marker:EZbiolab; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Bacterial vector for DNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22453276
Comments: Please cite the following article in any publications where this construct is utilized:
E.Y.D. Chua, D. Vasudevan, G.E. Davey, B. Wu & C.A. Davey. 2012. The Mechanics Behind DNA Sequence-Dependent Properties of the Nucleosome. Nucleic Acids Res. 40(13): 6338-6352.
Proper citation: RRID:Addgene_158572 Copy
Vector Backbone Description: Backbone Marker:EZbiolab; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Bacterial vector for DNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22453276
Comments: Please cite the following article in any publications where this construct is utilized:
E.Y.D. Chua, D. Vasudevan, G.E. Davey, B. Wu & C.A. Davey. 2012. The Mechanics Behind DNA Sequence-Dependent Properties of the Nucleosome. Nucleic Acids Res. 40(13): 6338-6352.
Proper citation: RRID:Addgene_158573 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F.
Proper citation: RRID:Addgene_158628 Copy
Species: Synthetic
Genetic Insert: Apixaban-Binding Helical Bundle
Vector Backbone Description: Backbone Marker:genscript; Backbone Size:5641; Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32883865
Proper citation: RRID:Addgene_158627 Copy
Genetic Insert: Pex22(1-36)-mRuby2
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158589 Copy
Genetic Insert: tNCS-ePTS1
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158588 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: membrane localization signal-tagBFP-wrmScarlet11
Vector Backbone Description: Backbone Size:5350; Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33693628
Proper citation: RRID:Addgene_158585 Copy
Vector Backbone Description: Vector Backbone:pXPR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606977
Comments: For questions regarding use of this plasmid, please contact John Doench.
Please visit https://www.biorxiv.org/content/10.1101/2020.05.17.100818v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158581 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pLX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606977
Comments: For questions regarding use of this plasmid, please contact David Root.
Please visit https://www.biorxiv.org/content/10.1101/2020.05.17.100818v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158580 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #25737; Vector Backbone:pSLIK hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. For pSLIK vectors, it is recommended to use zeocin in addition to ampicillin for bacterial selection.
Proper citation: RRID:Addgene_158639 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #25737; Vector Backbone:pSLIK hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). For pSLIK vectors, it is recommended to use zeocin in addition to ampicillin for bacterial selection.
Proper citation: RRID:Addgene_158635 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #25737; Vector Backbone:pSLIK hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. For pSLIK vectors, it is recommended to use zeocin in addition to ampicillin for bacterial selection.
Proper citation: RRID:Addgene_158636 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.
Proper citation: RRID:Addgene_158633 Copy
Genetic Insert: VioA
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158598 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
Proper citation: RRID:Addgene_158632 Copy
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