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Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA, tagged with a single epitope in the third extracellular loop (at an introduced NheI site) was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16812 Copy
Species: Synthetic
Genetic Insert: PAM for AvCAST + AvPSP1
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168146 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST TnsC
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168149 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST right end (RE)
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168145 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168140 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168141 Copy
Species: Synthetic
Genetic Insert: ymVenus
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_168058 Copy
Species: Synthetic
Genetic Insert: ymScarletI
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Comments: Contains the linker region at the 5’ end of the FP, to use with the standard F5 forward primer (GGTGACGGTGCTGGTTTA).
Proper citation: RRID:Addgene_168055 Copy
Species: Synthetic
Genetic Insert: Blue light-inducible AraC dimers in Escherichia coli
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33903769
Proper citation: RRID:Addgene_168050 Copy
Species: Synthetic
Genetic Insert: Blue light-inducible AraC dimers in Escherichia coli
Vector Backbone Description: Vector Backbone:pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33903769
Proper citation: RRID:Addgene_168051 Copy
Species: Homo sapiens
Genetic Insert: PGBD5
Vector Backbone Description: Backbone Marker:Genecopeia; Backbone Size:8721; Vector Backbone:pReceiver-Lv103; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504702
Proper citation: RRID:Addgene_168053 Copy
Species: Synthetic
Genetic Insert: mGreenLantern-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41919951
Proper citation: RRID:Addgene_168060 Copy
Species: Homo sapiens
Genetic Insert: Anticalin
Vector Backbone Description: Backbone Size:5222; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34918516
Proper citation: RRID:Addgene_168046 Copy
Species: Synthetic
Genetic Insert: ymTq2-yeVenus
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168076 Copy
Species: Synthetic
Genetic Insert: cyterm-mVenus
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_168079 Copy
Species: Synthetic
Genetic Insert: ystagRFP-T2A-mTq2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41919951
Proper citation: RRID:Addgene_168072 Copy
Species: Synthetic
Genetic Insert: mAvicFP1-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168073 Copy
Species: Synthetic
Genetic Insert: ymTq2-mAvicFP1
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41919951
Proper citation: RRID:Addgene_168075 Copy
Species: S.aureus
Genetic Insert: Cas9-P2A-mCherry
Vector Backbone Description: Backbone Marker:Addgene #40973; Vector Backbone:PBCAG-eGFP; Vector Types:Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288582
Comments: Puromycin selection disappears following Cre recombination.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.27.445896v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_168107 Copy
Species: Homo sapiens
Genetic Insert: HA-nEPEA-(EAAAK)3-OGA(544-706)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5352; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33686291
Proper citation: RRID:Addgene_168102 Copy
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