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Species: Synthetic
Genetic Insert: VAMP1(T29-K89)-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168010 Copy
Species: Synthetic
Genetic Insert: SNAP25-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168011 Copy
Species: Synthetic
Genetic Insert: SNAP25-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168013 Copy
Species: Synthetic
Genetic Insert: Dre recombinase
Vector Backbone Description: Vector Backbone:UC57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167968 Copy
Species: Synthetic
Genetic Insert: VAMP1(T29-K89)-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168008 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_167955 Copy
Species: Synthetic
Genetic Insert: Ykt6(L161-L177)-T3'PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168003 Copy
Species: Synthetic
Genetic Insert: PTEN-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168005 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz1-HA-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.
Comments: Rfz1 from Henk Roelink. This sequence has a few differences.
from the published sequence. Sites listed in Rfz1 are not a complete list.
and I haven't searched through the CS2+ sequence to see if/where those sites.
are present. HA tag version made by Jeff Brown (2/98-3/98) insert the epitope.
into the StyI site (1904). The 3' Sty site is destroyed by the tags, but.
the 5' site is inteact. Epitope can be detected in PBS/Formaldehyde fixed.
tissue permeablized with 0.2% Triton. Frizzled tends to ppt out of SDS sample.
buffer when boiled, so either warm samples to ~70 degrees Celsius for a.
few minutes before loading samples or use a strong denaturing loading buffer.
and incubate the samples at room temp for half and hour before loading.
The StyI site is in the 3rd extracellular loop of the protein.
Proper citation: RRID:Addgene_16797 Copy
Species: Synthetic
Genetic Insert: PTEN-T3'PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168006 Copy
Species: Rattus norvegicus
Genetic Insert: beta 2 AR Fz2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588714
Comments: Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-2 (intracellular). Activated by isoproterenol and inhibited by propranolol.
Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.
Proper citation: RRID:Addgene_16794 Copy
Species: Synthetic
Genetic Insert: VAMP7-T3'PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168002 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y717E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_167959 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168157 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168159 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168154 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST minimal CRISPR array
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168156 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST TnsD
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168150 Copy
Species: Rattus norvegicus
Genetic Insert: Fz1-ER-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1, and GFP at the C terminus.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16815 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The the insert was published by Wang et al., JBC 271: 4468 (1996), and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16813 Copy
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