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Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167890 Copy
Species: Synthetic
Genetic Insert: dxCas9(3.7)-VPR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: dxCas9(3.7)-VPR is cloned from dxCas(3.7)-VPR (Addgene Plasmid #108383).
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167937 Copy
Species: Synthetic
Genetic Insert: KRAB-dCas9-DHFR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: KRAB-dCas9 cloned from pAAVS1-NDi-CRISPRi (Gen1) (Addgene Plasmid #73497).
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167935 Copy
Species: Homo sapiens
Genetic Insert: CORO6
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: SGC link: http://www.thesgc.org/structures/7KYX/
Proper citation: RRID:Addgene_167845 Copy
Species: Homo sapiens
Genetic Insert: WDR55
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: SGC link: http://www.thesgc.org/structures/7KQQ/
Proper citation: RRID:Addgene_167844 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I and transcribe with SP6.
Plasmid History:
Constructed by Randall T. Moon.
Notes for Use:
Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.
Proper citation: RRID:Addgene_16788 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-PDZ
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10427095
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996)
was engineered to now contain Dsh amino acids 1-267 and 381-736 (deleting 268-380).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16786 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-COOH
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10427095
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-588 (deleting 599-736).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16784 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10, nsp16
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33423577
Comments: SGC clone sample ID: nsp16_SARS2-nsp10_SARS2-MVC019-A07:C245477
Proper citation: RRID:Addgene_167849 Copy
Species: Synthetic
Genetic Insert: EKAREN4(TA)
Vector Backbone Description: Vector Backbone:pLV lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis.
Proper citation: RRID:Addgene_167830 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32895498
Proper citation: RRID:Addgene_167838 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32895498
Proper citation: RRID:Addgene_167837 Copy
Species: Homo sapiens
Genetic Insert: TETv4 (TET1CD-XTEN80-dCas9)
Vector Backbone Description: Backbone Size:5574; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33838111
Proper citation: RRID:Addgene_167983 Copy
Species: Mus musculus
Genetic Insert: mFZ6-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43319), was used as template to PCR amplify just the coding region as an EcoR1 insert. The 3' stop was kept.
Constructed by: Rebecca Bates/Randall Moon.
Notes for Use:
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16802 Copy
Species: Homo sapiens
Genetic Insert: CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
Vector Backbone Description: Backbone Size:4836; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33838111
Proper citation: RRID:Addgene_167981 Copy
Species: Mus musculus
Genetic Insert: FZ7
Vector Backbone Description: Backbone Size:4095; Vector Backbone:PCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: 3 Kb 5' EcoRI - 3' XbaI insert of mFZ7/pRK5 (Nathans), with engineered optimal kozak sequence.
Plasmid History:
Species of Sequence Origin: Xenopus.
Constructed by: Rebecca Bates/Randall Moon.
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16801 Copy
Species: Mus musculus
Genetic Insert: Hrasls3
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-IPU-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_167987 Copy
Species: Homo sapiens
Genetic Insert: Anticalin
Vector Backbone Description: Backbone Size:5222; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34918516
Proper citation: RRID:Addgene_168039 Copy
Species: Synthetic
Genetic Insert: eCFP-Ykt6(Q131-K190)-mVenus
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:pET (pBR322); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33602850
Proper citation: RRID:Addgene_168030 Copy
Species: Synthetic
Genetic Insert: eCFP-Sec22b(A131-H190)-mVenus
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:pET (pBR322); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33602850
Comments: Please note: Plasmid contains a T186N mutation in mVenus. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_168029 Copy
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