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Species: Synthetic
Genetic Insert: Dummy_[Himar]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_225801 Copy
Species: Synthetic
Genetic Insert: V5-mScarlet-I
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224487 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies.
Proper citation: RRID:Addgene_201003 Copy
Species: Synthetic
Genetic Insert: V5-mTagBFP2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224484 Copy
Species: Synthetic
Genetic Insert: rox-3xNLS-mCherry-V5-2xSTOP-rox
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224524 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5253; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies.
Proper citation: RRID:Addgene_201002 Copy
Species: Synthetic
Genetic Insert: Golgi-mTagBFP-P2A-H2A-iRFP
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224490 Copy
Species: Synthetic
Genetic Insert: CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215261 Copy
Species: Synthetic
Genetic Insert: PDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215259 Copy
Species: Synthetic
Genetic Insert: pMAPKKK18:HISPS.TNOS-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215272 Copy
Species: Synthetic
Genetic Insert: Etr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_alpha1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215264 Copy
Species: Synthetic
Genetic Insert: CBS-att-OCS-HispS-tNos-SF-CPH-SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215268 Copy
Species: Synthetic
Genetic Insert: Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224497 Copy
Species: Synthetic
Genetic Insert: P35S:HISPS-SF-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215274 Copy
Species: Synthetic
Genetic Insert: PNOS:HISPS-SF-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215273 Copy
Species: Synthetic
Genetic Insert: TIRs of OposCharlie2 transposon
Vector Backbone Description: Vector Backbone:pMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38843831
Proper citation: RRID:Addgene_217098 Copy
Species: Synthetic
Genetic Insert: TIRs of S2_DM transposon
Vector Backbone Description: Vector Backbone:pMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38843831
Proper citation: RRID:Addgene_217090 Copy
Species: Synthetic
Genetic Insert: Mariner2_AG transposase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38843831
Proper citation: RRID:Addgene_217135 Copy
Species: Synthetic
Genetic Insert: Tc1-5_Xt transposase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38843831
Proper citation: RRID:Addgene_217132 Copy
Species: Synthetic
Genetic Insert: Tc1-1_AG transposase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38843831
Proper citation: RRID:Addgene_217137 Copy
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