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Species: Synthetic
Genetic Insert: 35s(ΔC_CRE)
Vector Backbone Description: Vector Backbone:pMK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32856047
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.14.095406v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_154601 Copy
Species: Escherichia coli
Genetic Insert: his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167651 Copy
Species: Escherichia coli
Genetic Insert: cba-his6
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The His6 tag is located at the C-terminus of colicin B. The parent plasmid is pKP579 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167658 Copy
Species: Homo sapiens
Genetic Insert: LHX6
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167779 Copy
Species: Escherichia coli
Genetic Insert: cba-his6 V21C, M261C
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2546922
Comments: parent plasmid is pKP579
Proper citation: RRID:Addgene_167657 Copy
Species: Homo sapiens
Genetic Insert: LHX2
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167776 Copy
Species: Synthetic
Genetic Insert: EKAREN5(TA)
Vector Backbone Description: Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774.
Proper citation: RRID:Addgene_167819 Copy
Species: Synthetic
Genetic Insert: EKAREN5
Vector Backbone Description: Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774.
Proper citation: RRID:Addgene_167818 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167686 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUBQ4-2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167685 Copy
Species: Staphylococcus aureus
Genetic Insert: pEC1.2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167684 Copy
Species: Staphylococcus aureus
Genetic Insert: pEC1.2::asCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167683 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUBQ4-2::asCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167682 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: HA tagged Xenopus Dishevelled with a point mutation in the binding pocket of the PDZ binding domain, that blocks binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16780 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167680 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167673 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUB4-2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: in addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167678 Copy
Species: Homo sapiens
Genetic Insert: STA5A
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167799 Copy
Species: Homo sapiens
Genetic Insert: SP7
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167798 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167672 Copy
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