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Species: Homo sapiens
Genetic Insert: 3x MHC I KB element - luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7707523
Comments: The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).
Proper citation: RRID:Addgene_26699 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, reporter for Rho; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15684032
Comments: The RhoA-binding domain of rhotekin (rGBD) was fused to eGFP in order to visualize active RhoA
Proper citation: RRID:Addgene_26732 Copy
Species: Kaposi's Sarcoma Associated Herpesvirus (HHV-8)
Genetic Insert: ORF36
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838604
Comments: ORF36 accession no.: AF148805
Proper citation: RRID:Addgene_26696 Copy
Species: Kaposi's Sarcoma Associated Herpesvirus (HHV-8)
Genetic Insert: ORF36
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838604
Comments: ORF36 accession no.: AF148805
Proper citation: RRID:Addgene_26695 Copy
Species: Epstein Bar Virus (EBV)
Genetic Insert: BGLF4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838604
Comments: BGLF4 Accession No.: DQ279927
Proper citation: RRID:Addgene_26691 Copy
Species: C. familiaris (dog RNAi)
Genetic Insert: dog Rab8b RNAi
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20890297
Comments: Dog Rab8b RNAi. Only matches dog. RNAi sequence is: 5'-GCGGGCAGAGCCAGGAATT-3'
Proper citation: RRID:Addgene_26708 Copy
Species: C. familiaris (dog RNAi)
Genetic Insert: dog Tuba RNAi
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20890297
Comments: RNAi to dog Tuba, but also matches multiple species, including human. RNAi target sequence is: 5'-GGAAATATGCAGATGGTGA-3'.
Proper citation: RRID:Addgene_26717 Copy
Species: C. familiaris (dog RNAi)
Genetic Insert: Dog Sec15A RNAi
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20890297
Comments: RNAi only matches dog Sec15A, does not match Sec15B. Does not match other species' Sec15A. RNAi target sequence is: 5'-GCACGGGTCATGATAGTTT-3'.
Proper citation: RRID:Addgene_26716 Copy
Species: Mus musculus
Genetic Insert: Actin-binding Protein-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19910490
Proper citation: RRID:Addgene_26719 Copy
Species: C. familiaris (dog RNAi)
Genetic Insert: dog Tuba RNAi
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20890297
Comments: RNAi to Dog Tuba (matches dog only). RNAi target sequence is: 5'-GCAGAGAAGTTAAAGGACA-3'.
Proper citation: RRID:Addgene_26718 Copy
Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20701745
Proper citation: RRID:Addgene_26679 Copy
Species: Homo sapiens
Genetic Insert: Rs1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4879; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21375737
Comments: pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences
Proper citation: RRID:Addgene_26797 Copy
Species: C. familiaris (dog RNAi)
Genetic Insert: dog Rab25 RNAi
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20890297
Comments: Matches dog and Panda Rab25. RNAi sequence is: 5'-AGAGATCTTCACCAAAGTG-3'
Proper citation: RRID:Addgene_26711 Copy
Genetic Insert: multiple cloning sequence
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21375737
Comments: Empty entry vector carrying Gateway R3L2 sites, with MCS. This plasmid can act as an empty carrier when combined with a L1L3 plasmid to generate expression plasmids carrying just the L1L3 component.
This plasmid was constructed as follows: The pEntr2B entry vector was digested with AflII and XhoI to remove the attL1 and ccdb genes. Oligos
containing the attR3 site flanked by AflII on the 5' end and a polylinker (NotI-PacI-Bsu36I-NdeI-XhoI) on the 3' end were ligated to generate pEntR3L2-MCS.
Proper citation: RRID:Addgene_26799 Copy
Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20701745
Comments: S73N is a reported SNP in the KPNA1 sequence. For more information, please see: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?type=rs&rs=4678193
Proper citation: RRID:Addgene_26677 Copy
Genetic Insert: multiple cloning sequence
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21375737
Comments: Empty entry vector carrying Gateway L1L3 sites, with MCS. This plasmid can act as an empty carrier when combined with a R3L2 plasmid to generate expression plasmids carrying just the R3L2 component (i.e., TetO-transgene expressing plasmid)
Plasmid was constructed as follows: The pEntr2B entry vector (Invitrogen) was digested with PstI and XhoI to remove the attL2 site.
Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side
and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites. A LoxP sequence was introduced at the XhoI site.
Proper citation: RRID:Addgene_26798 Copy
Genetic Insert: GFP-ZFN1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: The original paper describing this plasmid should also be cited: Pruett-Miller, S.M., Connelly, J.P., Maeder, M.L., Joung, J.K., and Porteus, M.H. (2008). Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol. Ther. 16, 707–717.
Proper citation: RRID:Addgene_26791 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20551166
Comments: H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector.
Proper citation: RRID:Addgene_26790 Copy
Species: Homo sapiens
Genetic Insert: Guanidinoacetate N-methyltransferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5404; Vector Backbone:pGL4.20; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917247
Comments: There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid.
Proper citation: RRID:Addgene_26804 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20729842
Proper citation: RRID:Addgene_26768 Copy
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