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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
p1242 3x-KB-L
 
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10+ mentions
RRID:Addgene_26699 3x MHC I KB element - luciferase Homo sapiens Ampicillin PMID:7707523 The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII). Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:05 21
GFP-rGBD
 
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10+ mentions
RRID:Addgene_26732 rhotekin Mus musculus Ampicillin PMID:15684032 The RhoA-binding domain of rhotekin (rGBD) was fused to eGFP in order to visualize active RhoA Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, reporter for Rho; Bacterial Resistance:Ampicillin RhoA binding domain of rhotekin Addgene 2026-10-17 02:44:05 13
KSHV ORF36 KD
 
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RRID:Addgene_26696 ORF36 Kaposi's Sarcoma Associated Herpesvirus (HHV-8) Ampicillin PMID:20838604 ORF36 accession no.: AF148805 Backbone Size:5000; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Kinase deficient mutant Lys 108 mutated to Gln Addgene 2026-10-17 02:44:05 1
KSHV ORF36
 
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RRID:Addgene_26695 ORF36 Kaposi's Sarcoma Associated Herpesvirus (HHV-8) Ampicillin PMID:20838604 ORF36 accession no.: AF148805 Backbone Size:5000; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:05 2
EBV BGLF4
 
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1+ mentions
RRID:Addgene_26691 BGLF4 Epstein Bar Virus (EBV) Ampicillin PMID:20838604 BGLF4 Accession No.: DQ279927 Backbone Size:5000; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:05 2
pLKO.1-blast-cfRab8b_1
 
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RRID:Addgene_26708 dog Rab8b RNAi C. familiaris (dog RNAi) Ampicillin PMID:20890297 Dog Rab8b RNAi. Only matches dog. RNAi sequence is: 5'-GCGGGCAGAGCCAGGAATT-3' Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin Dog Rab8b RNAi Addgene 2026-10-17 02:44:05 0
pLKO.1-blast-cfTuba_1
 
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RRID:Addgene_26717 dog Tuba RNAi C. familiaris (dog RNAi) Ampicillin PMID:20890297 RNAi to dog Tuba, but also matches multiple species, including human. RNAi target sequence is: 5'-GGAAATATGCAGATGGTGA-3'. Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin Dog Tuba RNAi Addgene 2026-10-17 02:44:05 0
pLKO.1-puro-cfSec15A_5
 
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RRID:Addgene_26716 Dog Sec15A RNAi C. familiaris (dog RNAi) Ampicillin PMID:20890297 RNAi only matches dog Sec15A, does not match Sec15B. Does not match other species' Sec15A. RNAi target sequence is: 5'-GCACGGGTCATGATAGTTT-3'. Backbone Size:7000; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin Dog Sec15A-specific RNAi. Addgene 2026-10-17 02:44:05 0
pEGFPc2 mAbp1
 
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1+ mentions
RRID:Addgene_26719 Actin-binding Protein-1 Mus musculus Kanamycin PMID:19910490 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:05 2
pLKO.1-blast-cfTuba_2
 
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RRID:Addgene_26718 dog Tuba RNAi C. familiaris (dog RNAi) Ampicillin PMID:20890297 RNAi to Dog Tuba (matches dog only). RNAi target sequence is: 5'-GCAGAGAAGTTAAAGGACA-3'. Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin Canine Tuba_2 RNAi Addgene 2026-10-17 02:44:05 0
pCMVTNT-T7-KPNA3
 
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1+ mentions
RRID:Addgene_26679 Karyopherin Alpha 3 Homo sapiens Ampicillin PMID:20701745 Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:05 5
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
 
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RRID:Addgene_26797 Rs1 Homo sapiens Ampicillin PMID:21375737 pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences Backbone Marker:Invitrogen; Backbone Size:4879; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D100A Addgene 2026-10-17 02:44:06 0
pLKO.1-blast-cfRab25_2
 
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RRID:Addgene_26711 dog Rab25 RNAi C. familiaris (dog RNAi) Ampicillin PMID:20890297 Matches dog and Panda Rab25. RNAi sequence is: 5'-AGAGATCTTCACCAAAGTG-3' Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin RNAi to canine Rab25. Addgene 2026-10-17 02:44:05 0
pEnt R3L2-MCS
 
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RRID:Addgene_26799 multiple cloning sequence Kanamycin PMID:21375737 Empty entry vector carrying Gateway R3L2 sites, with MCS. This plasmid can act as an empty carrier when combined with a L1L3 plasmid to generate expression plasmids carrying just the L1L3 component. This plasmid was constructed as follows: The pEntr2B entry vector was digested with AflII and XhoI to remove the attL1 and ccdb genes. Oligos containing the attR3 site flanked by AflII on the 5' end and a polylinker (NotI-PacI-Bsu36I-NdeI-XhoI) on the 3' end were ligated to generate pEntR3L2-MCS. Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:06 0
pCMVTNT-T7-KPNA1
 
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1+ mentions
RRID:Addgene_26677 Karyopherin Alpha 1 Homo sapiens Ampicillin PMID:20701745 S73N is a reported SNP in the KPNA1 sequence. For more information, please see: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?type=rs&rs=4678193 Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin KPNA1 sequence contains a reported SNP at S73N Addgene 2026-10-17 02:44:05 7
pEnt L1L3-MCS
 
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RRID:Addgene_26798 multiple cloning sequence Kanamycin PMID:21375737 Empty entry vector carrying Gateway L1L3 sites, with MCS. This plasmid can act as an empty carrier when combined with a R3L2 plasmid to generate expression plasmids carrying just the R3L2 component (i.e., TetO-transgene expressing plasmid) Plasmid was constructed as follows: The pEntr2B entry vector (Invitrogen) was digested with PstI and XhoI to remove the attL2 site. Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites. A LoxP sequence was introduced at the XhoI site. Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:06 0
GFPZFN1.4-B2H
 
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1+ mentions
RRID:Addgene_26791 GFP-ZFN1 Ampicillin PMID:19540188 The original paper describing this plasmid should also be cited: Pruett-Miller, S.M., Connelly, J.P., Maeder, M.L., Joung, J.K., and Porteus, M.H. (2008). Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol. Ther. 16, 707–717. Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:06 2
pBABE-H2BGFP
 
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10+ mentions
RRID:Addgene_26790 H2B-GFP Homo sapiens Ampicillin PMID:20551166 H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector. Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:06 12
-4200GAMT-P1-pGL4
 
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RRID:Addgene_26804 Guanidinoacetate N-methyltransferase Homo sapiens Ampicillin PMID:19917247 There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid. Backbone Marker:Promega; Backbone Size:5404; Vector Backbone:pGL4.20; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:06 0
pShuttle mCherry-Tubulin
 
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1+ mentions
RRID:Addgene_26768 Tubulin Homo sapiens Kanamycin PMID:20729842 Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:06 5

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