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Species: Saccharomyces cerevisiae
Genetic Insert: MAT Z1
Vector Backbone Description: Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Addgene QC identified differences to the theoretical sequence that the depositing lab does not believe are of functional concern.
Proper citation: RRID:Addgene_166099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL2
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:291472..291491 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166088 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP1-1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVIII:212613..212632 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166086 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET16
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXVI:877523..877542 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome.
Addgene QC identified several discrepancies compared to the theoretical reference.
Proper citation: RRID:Addgene_166091 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RPL22A
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:263006..263025 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome.
Addgene QC identified discrepancies compared to the theoretical sequence. The depositing lab confirms the functional activity of the construct despite these differences.
Proper citation: RRID:Addgene_166079 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RPL39 (Overlaps with 5'UTR and first base of gene)
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrX: 75914-75933 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166078 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SLA1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII: 213734-213753 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166075 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of RRT8
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXV:241445..241464 (-strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166080 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Msn2-sgRNA#27
Vector Backbone Description: Vector Backbone:pYTK101; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166103 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAT Z1
Vector Backbone Description: Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166100 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Msn2-sgRNA#27
Vector Backbone Description: Vector Backbone:pYTK103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Murine polyomavirus deltaVP1 (NLS deletion mutant)
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166675 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CAK1 cyclin-dependent protein kinase-activating kinase CAK1
Vector Backbone Description: Backbone Size:5022; Vector Backbone:pLIB-HIS-TEV; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791727
Proper citation: RRID:Addgene_177014 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NHX1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177108 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ANP1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177100 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MNN9
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NHX1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177107 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE13
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+6
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17719 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+8
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17721 Copy
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