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Species: Drosophila melanogaster
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531964
Proper citation: RRID:Addgene_174421 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174495 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27473911
Comments: For protein expression, grow at 17degC.
Plasmid used in the following publication:
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
Proper citation: RRID:Addgene_174496 Copy
Species: Drosophila melanogaster
Genetic Insert: Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28395731
Comments: To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCherry: Clonetech
Proper citation: RRID:Addgene_163926 Copy
Species: Drosophila melanogaster
Genetic Insert: Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Vector Backbone Description: Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30079589
Proper citation: RRID:Addgene_164153 Copy
Species: Drosophila melanogaster
Genetic Insert: Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Vector Backbone Description: Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30079589
Proper citation: RRID:Addgene_164148 Copy
Species: Drosophila melanogaster
Genetic Insert: Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Vector Backbone Description: Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30079589
Proper citation: RRID:Addgene_164149 Copy
Species: Drosophila melanogaster
Genetic Insert: U6 and DR
Vector Backbone Description: Backbone Size:7350; Vector Backbone:white+attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616113
Proper citation: RRID:Addgene_164586 Copy
Species: Drosophila melanogaster
Genetic Insert: Egl-Trbo
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pUASp-attB-K10; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35020877
Proper citation: RRID:Addgene_179993 Copy
Species: Drosophila melanogaster
Genetic Insert: mutant Odd Skipped
Vector Backbone Description: Backbone Size:3633; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18332042
Proper citation: RRID:Addgene_18044 Copy
Species: Drosophila melanogaster
Genetic Insert: Hel25E
Vector Backbone Description: Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29773557
Proper citation: RRID:Addgene_110123 Copy
Species: Drosophila melanogaster
Genetic Insert: Hel25E
Vector Backbone Description: Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29773557
Proper citation: RRID:Addgene_110122 Copy
Species: Drosophila melanogaster
Genetic Insert: dati
Vector Backbone Description: Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29773557
Comments: Depositor notes discrepancies found in Addgene QC sequence are not of functional concern
Proper citation: RRID:Addgene_110119 Copy
Species: Drosophila melanogaster
Genetic Insert: U6.3-gRNA[DsxF]
Vector Backbone Description: Vector Backbone:white+attB; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30622266
Proper citation: RRID:Addgene_112690 Copy
Species: Drosophila melanogaster
Genetic Insert: U6.3-gRNA[bTub] & U6.3-gRNA[DsxF]
Vector Backbone Description: Vector Backbone:white+attB; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30622266
Proper citation: RRID:Addgene_112694 Copy
Species: Drosophila melanogaster
Genetic Insert: cort (Cdc20)
Vector Backbone Description: Vector Backbone:pUASp; Vector Types:P insertion vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18020708
Proper citation: RRID:Addgene_112991 Copy
Species: Drosophila melanogaster
Genetic Insert: SuUR
Vector Backbone Description: Vector Backbone:pGEX 4t2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25437540
Proper citation: RRID:Addgene_112990 Copy
Species: Drosophila melanogaster
Genetic Insert: gnu
Vector Backbone Description: Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28555567
Proper citation: RRID:Addgene_112994 Copy
Species: Drosophila melanogaster
Genetic Insert: full length gnu
Vector Backbone Description: Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29480805
Proper citation: RRID:Addgene_112999 Copy
Species: Drosophila melanogaster
Genetic Insert: pumilio
Vector Backbone Description: Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29480805
Comments: Note: Plasmid encodes Pum amino acids 566-1090 with a N-terminal GST tag
Proper citation: RRID:Addgene_112997 Copy
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