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Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170039 Copy
Species: Homo sapiens
Genetic Insert: FKBP DD (L106P)-UbK0G75S
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33007216
Proper citation: RRID:Addgene_170157 Copy
Genetic Insert: RESCUE-DDC-λN-NES
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170154 Copy
Species: Homo sapiens
Genetic Insert: BoxB-RAB7A(ACG)-MS2
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170155 Copy
Species: Mus musculus
Genetic Insert: catalase
Vector Backbone Description: Backbone Marker:Eric Kowarz; Addgene plasmid # 60495; Backbone Size:5400; Vector Backbone:pSBtet-GP; Vector Types:Mammalian Expression, genome integration via Sleeping Beauty transposon system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33981726
Proper citation: RRID:Addgene_170152 Copy
Species: Homo sapiens
Genetic Insert: NES-MCP-RESCUE-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170153 Copy
Species: Homo sapiens
Genetic Insert: chromodomain helicase DNA binding protein 7
Vector Backbone Description: Backbone Size:4776; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23134727
Proper citation: RRID:Addgene_170145 Copy
Species: Homo sapiens
Genetic Insert: chromodomain helicase DNA binding protein 7
Vector Backbone Description: Backbone Size:4776; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23134727
Proper citation: RRID:Addgene_170143 Copy
Genetic Insert: ChroME2s-ST-P2A-NLS-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_170177 Copy
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pGIlesSHdI; Vector Types:Unspecified; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_170048 Copy
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pGilesSHdI; Vector Types:Unspecified; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_170047 Copy
Species: Homo sapiens
Genetic Insert: EGFP-RSKL
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170165 Copy
Species: Homo sapiens
Genetic Insert: EGFP-SKL
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170166 Copy
Genetic Insert: ChroME2s-P2A-H2B-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_170163 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:13043; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Please note the OriP sequence contains a deletion that is also found in Addgene plasmid (pCEP4-CXCR4) which was used as the backbone.
Proper citation: RRID:Addgene_170041 Copy
Species: Homo sapiens
Genetic Insert: MAPK9
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170238 Copy
Species: Homo sapiens
Genetic Insert: CHUK
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Comments: Please note: Plasmid contains a V268I mutation in CHUK. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_170236 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170190 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170191 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170194 Copy
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