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Plasmids are provided by Addgene and DGRC.

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pET-PE2-His (IK1822)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_170103 bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis Synthetic Kanamycin PMID:33927418 This is a bacterial codon-optimized SpCas9 (H840A) sequence within pET-28b backbone (Addgene #73018) fused to bipartite NLS, a 33-amino acid linker and the engineered M-MLV RT from the parental pCMV-PE2 plasmid (Addgene #132775). To this construct, a short GS linker and a 6xHis-tag are added to C terminus. Backbone Size:5238; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin Bacteria codon-optimized nSpCas9 (H840A) & M-MLV RT (D200N, T306K, W313F, T330P, L603W) 2026-08-01 01:07:59 2
pT7RNAP
 
Resource Report
Resource Website 		RRID:Addgene_170101 T7-RNA polymerase Kanamycin PMID:34985758 Backbone Size:3262; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-01 01:07:59 0
pGFP-PacI
 
Resource Report
Resource Website 		RRID:Addgene_170100 deGFP Synthetic Ampicillin PMID:34985758 Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry
 
Resource Report
Resource Website 		RRID:Addgene_170183 sgBFP Ampicillin PMID:35076284 Backbone Marker:Fraser lab; Vector Backbone:pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:00 0
pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry
 
Resource Report
Resource Website 		RRID:Addgene_170184 Sa163 RT Stigmatella aurantica Ampicillin PMID:35076284 Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:08:00 0
All-in-one split-ADAR2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_170136 ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN Homo sapiens Kanamycin PMID:35044296 Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin 2026-08-01 01:08:00 1
pEcCas5
 
Resource Report
Resource Website 		RRID:Addgene_170090 E. coli type I-E Cas5 E. coli Kanamycin PMID:34985758 If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:07:59 0
pGFP-GTATT
 
Resource Report
Resource Website 		RRID:Addgene_170099 deGFP Synthetic Ampicillin PMID:34985758 Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
pGFP-CAATG
 
Resource Report
Resource Website 		RRID:Addgene_170097 deGFP Synthetic Ampicillin PMID:34985758 Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
pSpG-PPE
 
Resource Report
Resource Website 		RRID:Addgene_170130 nCas9-SpG(H840A)-M-MLV Mus musculus Ampicillin PMID:33767395 Vector Backbone:pUC; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R for Cas9; D200N, T306K, W313F, T330P and L603W for M-MLV 2026-08-01 01:08:00 0
pH-nCas9-PPE-V2
 
Resource Report
Resource Website 		RRID:Addgene_170131 nCas9(H840A)-M-MLV Mus musculus Kanamycin PMID:33767395 Backbone Marker:Qi-Jun Chen; Vector Backbone:pHUE411 (modified from pCambia); Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin H840A for Cas9; D200N, T306K, W313F, T330P and L603W for M-MLV 2026-08-01 01:08:00 0
pGFP-CAAAG
 
Resource Report
Resource Website 		RRID:Addgene_170096 deGFP Synthetic Ampicillin PMID:34985758 Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
pEcCse2
 
Resource Report
Resource Website 		RRID:Addgene_170094 E. coli type I-E Cse2 E. coli Kanamycin PMID:34985758 If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:07:59 0
MS2-adRNA (3'UAG)
 
Resource Report
Resource Website 		RRID:Addgene_170128 MS2-ADAR2(3'UAG)-MS2 Homo sapiens Ampicillin PMID:35044296 Backbone Marker:Addgene #52961; Backbone Size:10500; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:00 0
MS2-adRNA (5'UAG)
 
Resource Report
Resource Website 		RRID:Addgene_170126 MS2-ADAR2(5'UAG)-MS2 Homo sapiens Ampicillin PMID:35044296 Backbone Marker:Addgene #52961; Backbone Size:10500; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
PdnaK-IR2 GFP pZa
 
Resource Report
Resource Website 		RRID:Addgene_170081 Green fluorescent protein Bacteria Chloramphenicol PMID:31536326 Backbone Size:2076; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-01 01:07:59 0
pEc-crRNA1
 
Resource Report
Resource Website 		RRID:Addgene_170088 E. coli type I-E single spacer array Chloramphenicol PMID:34985758 Backbone Size:1688; Vector Backbone:ColE1; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-01 01:07:59 0
Circular 200,100 with 8 bp bulge loops (GAPDH)
 
Resource Report
Resource Website 		RRID:Addgene_170121 Circular 200,100 guide RNA with 8bp bulge loops Homo sapiens Kanamycin PMID:35145312 Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin 2026-08-01 01:07:59 0
Circular 200,100 (mPCSK9)
 
Resource Report
Resource Website 		RRID:Addgene_170122 Circular 200,100 guide RNA Mus musculus Ampicillin PMID:35145312 Backbone Marker:Addgene #78601; Backbone Size:5700; Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
PdnaK-IR3 GFP pZa
 
Resource Report
Resource Website 		RRID:Addgene_170084 Green fluorescent protein Bacteria Chloramphenicol PMID:31536326 Backbone Size:2078; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-01 01:07:59 0

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