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Species: Homo sapiens
Genetic Insert: HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32214 Copy
Species: Homo sapiens
Genetic Insert: HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32213 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech - Discontinued; Backbone Size:8100; Vector Backbone:pACT2 AD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32170 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: CEN4 ARS1 S. cerevisiae replication.
Ubiquitin was amplified using oligos which introduce BamHI sites on either end and cloned in to the BamHI site on the vector. Insertion was confirmed by digests and then orientation confirmed by sequencing.
Proper citation: RRID:Addgene_32173 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Wildtype STE2 ORF including 1100bp upstream of the start codon (from SalI site) and 200bp downstream of the stop codon (HindIII). The wildtype 1.6kB HindIII fragment encoding STE2 was subcloned from LHP123 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI (upstream)/PstI fragment and ligating in the 2kB EcoRI/PstI fragment from pJR3.
Proper citation: RRID:Addgene_32171 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: STE2 ORF with a stop codon introduced at aa378. The 1.6kB STE2 378Stop -encoding HindIII fragment was subcloned from LHP147 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI(upstream)/PstI fragment and ligating in the 2KB EcoRI/PstI fragment from pJR3.
Proper citation: RRID:Addgene_32172 Copy
Species: Homo sapiens
Genetic Insert: HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:37000; Vector Backbone:pAd CMV/V5 DEST; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32218 Copy
Species: Homo sapiens
Genetic Insert: HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32216 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb).
K63A mutation was introduced by site-directed mutagenesis of LHP585 (Addgene 32168).
2 µm S. cerevisiae replication.
Proper citation: RRID:Addgene_32166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: BglII-KpnI K48R/K63R fragment of synthetic yeast Ubiquitin cloned into YEp96 (D. Ecker, U PENN).
2 µm S. cerevisiae replication.
Proper citation: RRID:Addgene_32167 Copy
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21300640
Proper citation: RRID:Addgene_32200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: uba1 yeast
Vector Backbone Description: Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12628920
Comments: A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb).
2 µm S. cerevisiae replication.
Proper citation: RRID:Addgene_32168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.
K63R mutation introduced by quickchange of LHP308 (Addgene plasmid 32151).
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32160 Copy
Species: Homo sapiens
Genetic Insert: HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:37000; Vector Backbone:pAd CMV/V5 DEST; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32208 Copy
Genetic Insert: EnvARGCD
Vector Backbone Description: Vector Backbone:pCMMP-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17329205
Proper citation: RRID:Addgene_32206 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAN1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: PAN1-HA including 1kB upstream sequence and 280bp downstream sequence of PAN1 ORF.
Proper citation: RRID:Addgene_32159 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32158 Copy
Genetic Insert: TAL024
Vector Backbone Description: Backbone Size:2708; Vector Backbone:pUC57-ΔBsaI; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Proper citation: RRID:Addgene_32265 Copy
Genetic Insert: loxP> STOP STOP loxP>
Vector Backbone Description: Vector Backbone:pJFRC2-10XUAS-IVS-mCD8::GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21831835
Proper citation: RRID:Addgene_32145 Copy
Genetic Insert: TAL025
Vector Backbone Description: Backbone Size:2708; Vector Backbone:pUC57-ΔBsaI; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Proper citation: RRID:Addgene_32266 Copy
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