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Species: Synthetic
Genetic Insert: monomeric superfolder green fluorescent protein
Vector Backbone Description: Vector Backbone:pMRS-GFP; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin and Gentamicin
Defining Citation: PMID:39163848
Proper citation: RRID:Addgene_220202 Copy
Species: Synthetic
Genetic Insert: iCre(R297T)
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid alias: CN5508 . This work supported by NIH BRAIN Initiative grant UF1MH128339.
Proper citation: RRID:Addgene_224199 Copy
Species: Synthetic
Genetic Insert: CoChR-EGFP
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid alias: CN5356 . This work supported by NIH BRAIN Initiative grant UF1MH128339.
Proper citation: RRID:Addgene_224196 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221952 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221954 Copy
Species: Synthetic
Genetic Insert: H2B-mCerulean
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224543 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: Addgene's NGS results found a mixed population of A/T nucleotides at position 2,840 that may result in a N646I mutation in the insert. This site defines the specificity of a DNA-binding protein for one 3’-terminal base within a 15-bp-long sequence. The mutation's effect on the affinity of the protein should be minimal.
5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221959 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221956 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221955 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221957 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-DddA6/11 1397C-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: TALE-free control for DdCBE experiments in mammalian cells. 5' Cloning Site: (Not Destroyed), 3' Cloning Site: (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221943 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-DddAtox 1397N-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: TALE-free control for DdCBE experiments in mammalian cells. 5' Cloning Site: (Not Destroyed), 3' Cloning Site: (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221942 Copy
Species: Synthetic
Genetic Insert: ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
Vector Backbone Description: Backbone Marker:Richard Sherwood, Addgene #71485; Vector Backbone:pT2-U6 sgBbsI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39313558
Proper citation: RRID:Addgene_205633 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221948 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-DddA11 1397N-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: TALE-free control for DdCBE experiments in mammalian cells. 5' Cloning Site: (Not Destroyed), 3' Cloning Site: (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221945 Copy
Species: Synthetic
Genetic Insert: COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39212664
Comments: 5' Cloning Site: BsmBI (Not Destroyed), 3' Cloning Site: BsmBI (Not Destroyed). Please visit https://www.biorxiv.org/content/10.1101/2024.05.13.593977v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_221947 Copy
Species: Synthetic
Genetic Insert: AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34506722
Comments: Insert was cloned via BsiWI (5') and AgeI (3', preserved in cloning) sites
Proper citation: RRID:Addgene_224443 Copy
Species: Synthetic
Genetic Insert: AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34506722
Comments: Insert was cloned via BsiWI (5') and AgeI (3', preserved in cloning) sites
Proper citation: RRID:Addgene_224440 Copy
Species: Synthetic
Genetic Insert: Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
Vector Backbone Description: Vector Backbone:pMRS-dCas9; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin and Gentamicin
Defining Citation: PMID:39163848
Proper citation: RRID:Addgene_220195 Copy
Species: Synthetic
Genetic Insert: Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
Vector Backbone Description: Vector Backbone:pMRS-dCas9; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin and Gentamicin
Defining Citation: PMID:39163848
Proper citation: RRID:Addgene_220196 Copy
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