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Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2416; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Red mCherry expression in the pharynx driven by the myo-3 promoter. unc-54 utr.
Proper citation: RRID:Addgene_19328 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mCherry
Vector Backbone Description: Backbone Size:2446; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use as co-injection marker. Red mCherry fluorescence in the pharynx. Do not use at high concentration (above 5 ng/ul) as it is toxic to animals.
Proper citation: RRID:Addgene_19327 Copy
Species: Caenorhabditis elegans
Genetic Insert: rme-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19382 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19379 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19378 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19391 Copy
Species: Caenorhabditis elegans
Genetic Insert: fog-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19390 Copy
Species: Caenorhabditis elegans
Genetic Insert: glp-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19393 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19392 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19386 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding A
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19300 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding B
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: Please note that this plasmid is actually quite toxic to cells. From the Sabatini lab:"it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells."
From the paper:
"To verify the actions of the Rags in a more physiological setting than that achieved by transient cDNA transfection, we generated HEK-293T cell lines stably expressing Rheb1, RagB, or RagBGTP (attempts to generate lines stably expressing RagBGDP failed)."
Proper citation: RRID:Addgene_19302 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding B
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19301 Copy
Species: Caenorhabditis elegans
Genetic Insert: cep-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19389 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding C
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19306 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding C
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19305 Copy
Species: Homo sapiens
Genetic Insert: Ras-related GTP binding D
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Proper citation: RRID:Addgene_19308 Copy
Species: Homo sapiens
Genetic Insert: WWP1 F792D
Vector Backbone Description: Backbone Marker:GEHealthcare; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16C
Proper citation: RRID:Addgene_179328 Copy
Species: Synthetic
Genetic Insert: mScarletI
Vector Backbone Description: Backbone Marker:none; Backbone Size:8200; Vector Backbone:pDB, derived from pUC57; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Proper citation: RRID:Addgene_179449 Copy
Species: Mus musculus
Genetic Insert: kappa opioid receptor
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34162205
Proper citation: RRID:Addgene_179285 Copy
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