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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
SauCas9 EMX1-nicking sgRNA
 
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RRID:Addgene_169860 SauCas9 EMX1-nicking sgRNA Ampicillin PMID:33837189 Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint. Backbone Size:600; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:57 0
Spy EMX1-pegRNA
 
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RRID:Addgene_169855 Spy EMX1-pegRNA Ampicillin PMID:33837189 Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint. Backbone Size:600; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:57 0
SauKKHCas9 PE2*
 
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RRID:Addgene_169852 SaCas9KKH-N580A-M-MLV Ampicillin PMID:33837189 Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint. Backbone Size:5517; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:57 0
SpyCas9 PE2*-3HA
 
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RRID:Addgene_169854 SpCas9-H840A-star-M-MLV-3HA Ampicillin PMID:33837189 Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint. Backbone Size:6635; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:57 0
SpyCas9 PE2*
 
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1+ mentions
RRID:Addgene_169850 spCas9 PE* Ampicillin PMID:33837189 Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint. Backbone Size:3400; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:57 1
U6-U57A
 
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RRID:Addgene_169927 U6 U57A Saccharomyces cerevisiae Ampicillin PMID:33547186 Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone. Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:58 0
U6-U57C
 
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RRID:Addgene_169928 U6 U57C Saccharomyces cerevisiae Ampicillin PMID:33547186 Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone. Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:58 0
pET-17b_K490clm_E215C
 
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RRID:Addgene_169995 kinesin family member 5B, isoform CRA_a Homo sapiens Ampicillin PMID:30770448 Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C7S, C65A, C168A, C174S, E215C, C294A, C330S, C421A 2026-08-01 01:07:58 0
pET-17b_Unc104-KIF5B-HALO
 
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RRID:Addgene_169996 Kinesin-like protein Unc-104 fused with KIF5B Homo sapiens Ampicillin PMID:32203385 Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:07:58 0
pKB232
 
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RRID:Addgene_170019 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
sgRNA.SFFV.tBFP
 
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RRID:Addgene_169940 Ampicillin Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:07:58 0
pKB226
 
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RRID:Addgene_170016 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
Bison sgRNA library
 
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1+ mentions
RRID:Addgene_169942 guide RNAs targeting E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes Ampicillin PMID:32494016 Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:07:58 1
pKB224
 
Resource Report
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RRID:Addgene_170014 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
pKB222
 
Resource Report
Resource Website
RRID:Addgene_170012 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0
pFastBac HTB DPP9S D808N
 
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RRID:Addgene_169947 DPP9 Homo sapiens Ampicillin PMID:33731932 Vector Backbone:pFastBac HTB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin D808N 2026-08-01 01:07:58 0
pcDNA3.1 LIC 6A CARD8-FLAG S297A
 
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RRID:Addgene_169949 CARD8 Homo sapiens Ampicillin PMID:34019797 Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S297A 2026-08-01 01:07:58 0
BCL6-GFP (1-275AA) in Cilantro
 
Resource Report
Resource Website
RRID:Addgene_169932 BCL6 1-275AA Homo sapiens Ampicillin PMID:33208943 https://benchling.com/s/seq-NeFKwxNgn6G0YAMn9Ym6 Vector Backbone:Cilantro 2 Plasmid #74450; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 160R>C 2026-08-01 01:07:58 0
BCL6-GFP R28A in Artichoke
 
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RRID:Addgene_169933 BCL6 R28A Homo sapiens Ampicillin PMID:33208943 https://benchling.com/s/seq-iwKB5ThUKLezYuBPsi9T Vector Backbone:Artichoke (Plasmid #73320); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 160R>C 2026-08-01 01:07:58 0
pAM005
 
Resource Report
Resource Website
RRID:Addgene_170009 Superfolder GFP Aequorea victoria Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-01 01:07:59 0

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