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Genetic Insert: H2B-APEX2
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pDEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478324
Proper citation: RRID:Addgene_107597 Copy
Species: Dugesia dorotocephala
Genetic Insert: opsin
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107630 Copy
Species: Danio rerio
Genetic Insert: sod1
Vector Backbone Description: Backbone Marker:4081; Vector Backbone:pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107594 Copy
Species: Dugesia dorotocephala
Genetic Insert: IFB
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107638 Copy
Species: Dugesia dorotocephala
Genetic Insert: sfrp-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107637 Copy
Species: Phagocata morgani
Genetic Insert: laminin
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107644 Copy
Species: Phagocata morgani
Genetic Insert: PC2
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107641 Copy
Species: Phagocata morgani
Genetic Insert: piwi-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107640 Copy
Species: Phagocata morgani
Genetic Insert: sfrp-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107649 Copy
Species: Phagocata morgani
Genetic Insert: ifb
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107647 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5922; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29666238
Comments: mCherry - 31% G+C% codon optimised for expression in Bacillus species
Proper citation: RRID:Addgene_107581 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mTagRFPT-CAAX at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_107580 Copy
Genetic Insert: pCDNA3.1hygro-BamHI-CLuc1433-NotI
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29535383
Comments: To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
Proper citation: RRID:Addgene_107611 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5788; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107577 Copy
Genetic Insert: NLucYAP15
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29535383
Comments: To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
Proper citation: RRID:Addgene_107610 Copy
Species: Rattus norvegicus
Genetic Insert: VGF
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107575 Copy
Species: Giardia sp.
Genetic Insert: porcupine-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107619 Copy
Species: Giardia sp.
Genetic Insert: opsin
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107618 Copy
Species: Giardia sp.
Genetic Insert: piwi-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107616 Copy
Vector Backbone Description: Vector Backbone:pSL1180; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11151300
Comments: Plasmid has an extensive polylinker (multiple cloning sites) flanked by two AscI and two FseI sites. It provides a convenient intermediate vector for cloning constructs into the transgenesis vectors described in Horn and Wimmer (2000), and into the Minos 3xP3 transgenesis vectors.
Proper citation: RRID:Addgene_107615 Copy
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