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http://www.addgene.org/63904

Vector Backbone Description: Backbone Size:5915; Vector Backbone:pYC44; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172

Proper citation: RRID:Addgene_63904 Copy   

•   Source: Addgene

http://www.addgene.org/63906

Vector Backbone Description: Backbone Size:5915; Vector Backbone:pYC44; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172

Proper citation: RRID:Addgene_63906 Copy   

•   Source: Addgene

http://www.addgene.org/63999

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63999 Copy   

•   Source: Addgene

http://www.addgene.org/63995

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63995 Copy   

•   Source: Addgene

http://www.addgene.org/63997

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63997 Copy   

•   Source: Addgene

http://www.addgene.org/64040

Species: Mus musculus
Genetic Insert: Pax8 paired domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17182173
Comments: There is a BamHI site in between the Eng and PDm coding sequences. Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function. Mouse Pax8 contains a P2A mutation. Depositor states that this mutation should not affect plasmid function.

Proper citation: RRID:Addgene_64040 Copy   

•   Source: Addgene

http://www.addgene.org/63991

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63991 Copy   

•   Source: Addgene

http://www.addgene.org/64047

Genetic Insert: gRNA
Vector Backbone Description: Backbone Size:9289; Vector Backbone:px548; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26355004
Comments: Confirmed by the Mendenhall and Myers labs to Flag epitope tag the endogenous human CEBPB gene when pooled with plasmids PX548_CEBPB_1 and pFETCH_CEPBP and transfected into human cells. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.

Proper citation: RRID:Addgene_64047 Copy   

•   Source: Addgene

http://www.addgene.org/63992

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63992 Copy   

•   Source: Addgene

http://www.addgene.org/64049

Species: Mus musculus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Comments: The c-Myc sequence was inserted into the region encoding the 1st exofacial loop of mouse GLUT4 in the background of pmCherry-N1. The fragment of Myc-GLUT4-mCherry was then sub-cloned into pLenti-hiko vector via NheI + HpaI digestion. Discrepancies between the Addgene QC sequence and the full sequence are not of functional concern. Please cite the following article: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).

Proper citation: RRID:Addgene_64049 Copy   

•   Source: Addgene

http://www.addgene.org/63994

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63994 Copy   

•   Source: Addgene

http://www.addgene.org/64048

Species: Saccharomyces cerevisiae
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Comments: The Lifeact sequence was initially reported in Riedl, J. et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods 5, 605–607 (2008). Please cite the following article as follows: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).

Proper citation: RRID:Addgene_64048 Copy   

•   Source: Addgene

http://www.addgene.org/64043

Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 Terminator 6..195 T K.l. URA3 Terminator 303..425 Degradation tag 426..476 K. lactis URA3 CDS without STOP codon 477..1277 K. lactis URA3 promoter 1278..1776 Consensus Ty2 3 1966..2152 pUC ori 2939..3582 Consensus Ty2 5 4987..5215 T ADH1 Terminator 5242..5436 pTEF1 Promoter 5455..5874 GFP CDS 5884..6600

Proper citation: RRID:Addgene_64043 Copy   

•   Source: Addgene

http://www.addgene.org/63990

Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766881
Comments: Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI

Proper citation: RRID:Addgene_63990 Copy   

•   Source: Addgene

http://www.addgene.org/64050

Species: Homo sapiens
Genetic Insert: AKT2
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Comments: The MYR sequence was inserted in frame at EcoRI/NheI site into N terminal of pLenti-FLAG-hAkt2 Please cite the following article: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).

Proper citation: RRID:Addgene_64050 Copy   

•   Source: Addgene

http://www.addgene.org/64053

Genetic Insert: see comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24452804
Comments: Escherichia coli MG1655 mutS_fs dnaG_Q576A exoX- xonA- xseA- 1255700::tolQRA Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-bla] tolC-

Proper citation: RRID:Addgene_64053 Copy   

•   Source: Addgene

http://www.addgene.org/64056

Species: Homo sapiens
Genetic Insert: RAD21 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCH_Donor; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid has been used along with PX548_RAD21_1 to add a FLAG tag to human RAD21 in HepG2 and MCF7 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.

Proper citation: RRID:Addgene_64056 Copy   

•   Source: Addgene

http://www.addgene.org/64060

Genetic Insert: tubulin
Vector Backbone Description: Vector Backbone:L304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23126280
Comments: Addgene sequencing results identified a duplication of the EGFP-Tubulin insert. It is not known how this impacts plasmid function.

Proper citation: RRID:Addgene_64060 Copy   

•   Source: Addgene

http://www.addgene.org/64063

Species: Mus musculus
Genetic Insert: bscl2
Vector Backbone Description: Vector Backbone:L309; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23458123

Proper citation: RRID:Addgene_64063 Copy   

•   Source: Addgene

http://www.addgene.org/64062

Species: Mus musculus
Genetic Insert: bscl2
Vector Backbone Description: Vector Backbone:L309; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23458123

Proper citation: RRID:Addgene_64062 Copy   

•   Source: Addgene