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Species: Synthetic
Genetic Insert: DNT3 DNT riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63852 Copy
Species: Synthetic
Genetic Insert: Theo45 theophylline riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63850 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9092628
Comments: AUG --> UUG start codon in lacZ
Proper citation: RRID:Addgene_63861 Copy
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pYC12; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63913 Copy
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pYC12; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63914 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a -1 frameshift mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63875 Copy
Vector Backbone Description: Backbone Marker:Zordan, et al, 2013; Backbone Size:4910; Vector Backbone:pGRB2.0; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63919 Copy
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pYC12; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63917 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63870 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63871 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63872 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63873 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1896445
Comments: pComb8 is nearly identical to the original pComb3 vector but contains the phage pVIII fusion protein for multi-valent expression along the sides of the phage via fusion with pVIII. It was designed for phage display of Fabs which are cloned into two separate cloning cassettes one chain at a time using SacI/XbaI restriction sites (for the light chain) and XhoI/SpeI restriction sites (for the heavy chain). The heavy chain is expressed fused to the pVIII. Both cassettes have the pelB leader sequence, the light chain and heavy chain-geneVIII fusion product assemble in the periplasm to form the Fab fragment. Soluble Fab can be expressed by removing the gene for the pVIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.
Proper citation: RRID:Addgene_63889 Copy
Species: Synthetic
Genetic Insert: ER-localized low-affinity GCaMP3(10.19)
Vector Backbone Description: Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC374 - pAAV EF1a DIO iRFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26451944
Proper citation: RRID:Addgene_63885 Copy
Vector Backbone Description: Backbone Size:4509; Vector Backbone:pMJ22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63920 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1896445
Comments: The original pComb3 vector was designed for phage display of Fabs which are cloned into two separate cloning cassettes one chain at a time using SacI/XbaI restriction sites (for the light chain) and XhoI/SpeI restriction sites (for the heavy chain). The heavy chain is expressed fused to the pVIII. Both cassettes have the pelB leader sequence, the light chain and heavy chain-geneIII fusion product assemble in the periplasm to form the Fab fragment. This vector system has also been used for the display of a wide variety of other proteins like zinc fingers, peptides, and cDNA fragments. Phage can be produced which express Fab or other proteins or peptides of interest fused to the phage pIII protein for expression on the head of the phage. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.
Proper citation: RRID:Addgene_63888 Copy
Vector Backbone Description: Backbone Size:4509; Vector Backbone:pMJ22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63928 Copy
Species: Mus musculus
Genetic Insert: Tet1 promoter fragment A
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25582196
Proper citation: RRID:Addgene_63881 Copy
Species: Mus musculus
Genetic Insert: Tet1 promoter fragment B
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25582196
Proper citation: RRID:Addgene_63882 Copy
Species: Mus musculus
Genetic Insert: Tet1 enhancer fragment E
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25582196
Proper citation: RRID:Addgene_63880 Copy
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