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Species: Homo sapiens
Genetic Insert: control gRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107402 Copy
Genetic Insert: kiwi-detecting toehold + sfGFP
Vector Backbone Description: Backbone Marker:Novagen 71406-3; Backbone Size:3340; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30083608
Proper citation: RRID:Addgene_107368 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting PBRM1
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain (Addgene plasmid # 21915); Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107401 Copy
Genetic Insert: banana-detecting toehold + sfGFP
Vector Backbone Description: Backbone Marker:Novagen 71406-3; Backbone Size:3340; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30083608
Proper citation: RRID:Addgene_107367 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting PBRM1
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain (Addgene plasmid # 21915); Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107400 Copy
Species: Homo sapiens
Genetic Insert: PBRM1
Vector Backbone Description: Vector Backbone:pTRIPZ-neo; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28082722
Comments: This plasmid is unstable and likely to recombine when grown. Upon receipt it is essential to screen multiple colonies to confirm the intact plasmid is present. Recombined products are not expected to impact viral packaging if the full length plasmid is present.
Proper citation: RRID:Addgene_107406 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting PBRM1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082722
Proper citation: RRID:Addgene_107404 Copy
Species: Homo sapiens
Genetic Insert: Dopamine Transporter (DAT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107457 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107456 Copy
Species: Escherichia coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29361543
Proper citation: RRID:Addgene_107455 Copy
Species: Escherichia Coli
Genetic Insert: bacterial mechanosensitive ion channel of large conductance
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, Adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29361543
Proper citation: RRID:Addgene_107454 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Met17
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: S. pombe Met17 gene is homologous to the S. cerevesiae MET17 gene.
Proper citation: RRID:Addgene_107453 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Met16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: S. pombe Met16 gene is homologous to the S. cerevesiae MET16 gene.
Proper citation: RRID:Addgene_107452 Copy
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A modified version of pSG5 (from Stratagene) in which a new polylinker has been cloned in.
Proper citation: RRID:Addgene_10737 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Proper citation: RRID:Addgene_10739 Copy
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29224783
Comments: Please note that the fluorescent protein encoded is dTomato, but this plasmid is named according to the associated publication and should function as described.
Proper citation: RRID:Addgene_107553 Copy
Vector Backbone Description: Backbone Marker:David Root; Backbone Size:8234; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: GFP can be used as an N-terminal tag, but the GFP can also be eliminated and used as a 'dropout' for cloning into the vector. NheI can be used to drop the GFP, and SalI can be used to keep GFP as an N-terminal tag. Its quite useful to have GFP as a tag as it can be used for localization, immunoprecipitation, and for Western Blotting.
Proper citation: RRID:Addgene_107551 Copy
Species: Escherichia coli
Genetic Insert: ftsB
Vector Backbone Description: Vector Backbone:pJKR-L-tetR; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30397005
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/04/314922 for bioRxiv preprint.
Proper citation: RRID:Addgene_107559 Copy
Species: Escherichia coli
Genetic Insert: ftsA
Vector Backbone Description: Vector Backbone:pJKR-L-tetR; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30397005
Comments: Please note that an IS4 transposase sequence was found between TetR and AmpR during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/05/04/314922 for bioRxiv preprint.
Proper citation: RRID:Addgene_107558 Copy
Genetic Insert: hMbCpf1(RR)
Vector Backbone Description: Backbone Size:5403; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30239882
Proper citation: RRID:Addgene_107533 Copy
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