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Species: Homo sapiens
Genetic Insert: b-catenin shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18483246
Comments: siRNA directed against beta-catenin: 5'-GCTTGGAATGAGACTGCTGAT-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Proper citation: RRID:Addgene_18803 Copy
Species: Homo sapiens
Genetic Insert: myc T58A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16094360
Proper citation: RRID:Addgene_18773 Copy
Species: Homo sapiens
Genetic Insert: H-Ras V12
Vector Backbone Description: Backbone Marker:Jay Morgenstern; Backbone Size:5763; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9054499
Proper citation: RRID:Addgene_18741 Copy
Species: Homo sapiens
Genetic Insert: H-Ras
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18744 Copy
Species: Homo sapiens
Genetic Insert: H-Ras V12 S35
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18746 Copy
Species: Homo sapiens
Genetic Insert: H-Ras V12 C40
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18747 Copy
Species: adenovirus
Genetic Insert: 12S E1A
Vector Backbone Description: Backbone Marker:Available at Addgene (#18750); Backbone Size:5915; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9054499
Proper citation: RRID:Addgene_18748 Copy
Vector Backbone Description: Backbone Marker:Jay Morgenstern; Backbone Size:5915; Vector Backbone:pWZL; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18750 Copy
Species: Mus musculus
Genetic Insert: 3 tandem zinc fingers
Vector Backbone Description: Backbone Size:3890; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18500337
Proper citation: RRID:Addgene_18752 Copy
Species: Mus musculus
Genetic Insert: 3 tandem zinc fingers
Vector Backbone Description: Backbone Size:3890; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18500337
Proper citation: RRID:Addgene_18753 Copy
Species: Mus musculus
Genetic Insert: zinc finger nuclease
Vector Backbone Description: Backbone Size:4760; Vector Backbone:CS2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18500337
Proper citation: RRID:Addgene_18754 Copy
Species: Mus musculus
Genetic Insert: zinc finger nuclease
Vector Backbone Description: Backbone Size:4760; Vector Backbone:CS2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18500337
Proper citation: RRID:Addgene_18755 Copy
Species: Mus musculus
Genetic Insert: 3 tandem zinc fingers
Vector Backbone Description: Backbone Size:3890; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18500337
Proper citation: RRID:Addgene_18756 Copy
Species: Mus musculus
Genetic Insert: thioredoxin-interacting protein
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16301999
Proper citation: RRID:Addgene_18758 Copy
Genetic Insert: Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17972876
Comments: The Author's Map is a representative Brainbow 1.1 construct (M= membrane tethered).
Please see attached PDF for important additional information.
Proper citation: RRID:Addgene_18722 Copy
Genetic Insert: hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17972876
Comments: The Author's Map is a representative Brainbow 2.1 construct (M= membrane tethered).
Please see attached PDF for important additional information.
Proper citation: RRID:Addgene_18723 Copy
Genetic Insert: dTomato ; EYFP ; mCerulean
Vector Backbone Description: Backbone Size:9188; Vector Backbone:pUC18+Thy1 promoter; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17972876
Comments: The Author's Map is a representative Brainbow 1.0 construct. The actual fluorescent genes are as indicated in the Gene/insert name.
Thy1 promoter. To linearize plasmid and remove vector sequences, EcoRI + PvuI , or alternatively NotI + PvuI are used (Note that PvuI also cuts inside the pUC18 vector).
Please see attached PDF for important additional information.
Proper citation: RRID:Addgene_18725 Copy
Species: Homo sapiens
Genetic Insert: pcDNA-DEST47 /hGLUT1-eGFP
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:7780; Vector Backbone:pcDNA-DEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18177733
Proper citation: RRID:Addgene_18729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1 L94A
Vector Backbone Description: Backbone Size:6268; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18692 Copy
Species: Homo sapiens
Genetic Insert: H-Ras
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18556515
Proper citation: RRID:Addgene_18693 Copy
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