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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pEZYmyc-His Resource Report Resource Website 1+ mentions |
RRID:Addgene_18701 | Chloramphenicol and Ampicillin | PMID:18064608 | pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag. | Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:38 | 6 | |||
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pEZY45 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18704 | Chloramphenicol and Ampicillin | PMID:18064608 | pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid. | Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:38 | 1 | |||
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pEZY202 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18705 | Chloramphenicol and Ampicillin | PMID:18064608 | pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid. | Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:38 | 1 | |||
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pGWF1 Resource Report Resource Website |
RRID:Addgene_18856 | Chloramphenicol and Ampicillin | PMID:18522753 | pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460. | Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:39 | 0 | |||
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pGC163 Resource Report Resource Website |
RRID:Addgene_19635 |
Gateway(R1-R2) |
Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:18723890 | Backbone Size:0; Vector Backbone:pGC160; Vector Types:Worm Expression, FLP/FRT, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:46 | 0 | ||
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pGC276 Resource Report Resource Website 1+ mentions |
RRID:Addgene_19637 |
Gateway(R1-R2) |
Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:18723890 | Sequence mismatches between Addgene sequence and depositor sequence do not affect function. | Backbone Size:0; Vector Backbone:pGC180; Vector Types:Worm Expression, FLP/FRT, destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:46 | 1 | |
|
pGC320 Resource Report Resource Website |
RRID:Addgene_19639 |
Gateway(R4-R3) |
Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:18723890 | Sequence mismatches between Addgene sequence and depositor sequence do not affect function. | Backbone Size:0; Vector Backbone:pGC246; Vector Types:Worm Expression, FLP/FRT, destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:47 | 0 | |
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pGC267 Resource Report Resource Website |
RRID:Addgene_19618 | Gateway(R1-R2)-iA-FLP::let-858(3’)-(Cbr)unc-119(+) | Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:18723890 | Backbone Size:0; Vector Backbone:pGC180; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:11:46 | 0 | ||
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pRosa26-DEST Resource Report Resource Website 10+ mentions |
RRID:Addgene_21189 | GateWay conversion cassette | Chloramphenicol and Ampicillin | PMID:19014667 | pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4 for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation. Stbl3 cells are advisable to use after users have done the LR recombination reaction. See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html for more information and protocols for this plasmid. Further advice from the depositing lab: "Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive... - Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay" Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct. | Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:00 | 18 | ||
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pCSDest Resource Report Resource Website 10+ mentions |
RRID:Addgene_22423 | Chloramphenicol and Ampicillin | PMID:17948311 | pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette | Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:12 | 17 | |||
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pCSDest2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_22424 | Chloramphenicol and Ampicillin | PMID:17948311 | pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3 | Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:11 | 7 | |||
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pSLIK-Venus Resource Report Resource Website 10+ mentions |
RRID:Addgene_25734 | None | Chloramphenicol and Ampicillin | PMID:16945906 | Parent lentivector, co-expression of Venus selection gene | Backbone Marker:Iain Fraser; Backbone Size:13170; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:47 | 10 | ||
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pSLIK-Zeo Resource Report Resource Website 1+ mentions |
RRID:Addgene_25736 | Chloramphenicol and Ampicillin | PMID:16945906 | Parent lentivector, co-expression of Zeocin selection gene | Backbone Marker:Iain Fraser; Backbone Size:12944; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:44 | 3 | |||
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pLX304 Resource Report Resource Website 100+ mentions |
RRID:Addgene_25890 | Chloramphenicol and Ampicillin | PMID:21706014 | There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence. | Backbone Size:9377; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:46 | 166 | |||
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pMBD-Gate1 Resource Report Resource Website |
RRID:Addgene_25946 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:50 | 0 | ||||
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pMAD-Gate4 Resource Report Resource Website |
RRID:Addgene_25943 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:49 | 0 | ||||
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pMAD-Gate6 Resource Report Resource Website |
RRID:Addgene_25945 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:46 | 0 | ||||
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pMAD-Gate2 Resource Report Resource Website |
RRID:Addgene_25941 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:46 | 0 | ||||
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pMBD-Gate3 Resource Report Resource Website |
RRID:Addgene_25959 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:50 | 0 | ||||
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pMBD-Gate4 Resource Report Resource Website |
RRID:Addgene_25960 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:46 | 0 |
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