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Species: Danio rerio
Genetic Insert: Foxj1a Promoter
Vector Backbone Description: Backbone Size:4464; Vector Backbone:pDestTol2pA2; Vector Types:MultiSite Gateway destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32312952
Proper citation: RRID:Addgene_163829 Copy
Species: Danio rerio
Genetic Insert: cytosolic phospholipase a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27203112
Proper citation: RRID:Addgene_163844 Copy
Species: Danio rerio
Genetic Insert: CD44b
Vector Backbone Description: Vector Backbone:pT3TS-Dest_R1-R3 (Addgene 140878); Vector Types:In vitro transcription of RNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_163850 Copy
Species: Danio rerio
Genetic Insert: Cavin1a
Vector Backbone Description: Vector Backbone:pDest-Tol2-pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_163852 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pT3TS-Dest_R1-R3 (Addgene 140878); Vector Types:In vitro transcription of RNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_163851 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pDest-Tol2-pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_163854 Copy
Species: Danio rerio
Genetic Insert: Cavin4a
Vector Backbone Description: Vector Backbone:pDest-Tol2-pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_163853 Copy
Species: Danio rerio
Genetic Insert: ythdf1
Vector Backbone Description: Vector Backbone:pSP64T; Vector Types:Production of mRNA for zebrafish injection.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378672
Proper citation: RRID:Addgene_164488 Copy
Species: Danio rerio
Genetic Insert: beta actin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7903; Vector Backbone:pDestTol2pA; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934216
Proper citation: RRID:Addgene_162069 Copy
Species: Danio rerio
Genetic Insert: cytosolic phospholipase a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27203112
Proper citation: RRID:Addgene_162566 Copy
Species: Danio rerio
Genetic Insert: cytosolic phospholipase a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27203112
Proper citation: RRID:Addgene_162568 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+.
Species of Sequence Origin: zebrafish.
Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.
Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_17072 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR.
Species of Sequence Origin: zebrafish.
Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.
Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_17074 Copy
Species: Danio rerio
Genetic Insert: vox-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93).
For RNA: cut with Asp718 and transcribe with SP6.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Constructed by: David Kimelman, 6/28/97 XIX p. 67.
Proper citation: RRID:Addgene_17064 Copy
Species: Danio rerio
Genetic Insert: Stbm
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: 6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert.
For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.
Proper citation: RRID:Addgene_17068 Copy
Species: Danio rerio
Genetic Insert: Dapper-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. cloned into Cla1/Xba1 sites.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17082 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. 1425bp EcoR1-Xba1 insert.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17075 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an kinase dead mutant of NLK1, analogous to K155M mutant of mouse NLK.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17078 Copy
Species: Danio rerio
Genetic Insert: Dvl-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled 2 deleted in amino acids 231-252, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut ASP718, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17087 Copy
Species: Danio rerio
Genetic Insert: Dvl-3
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled 3, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17089 Copy
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