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Species: Drosophila melanogaster
Genetic Insert: dme-miR-1003 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.
Proper citation: RRID:Addgene_17761 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-let-7 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin and cloned into pMT-puro.
Proper citation: RRID:Addgene_17764 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7 perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-ps (gagctcACTATACAACCTACTACCTCAactagt) Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17766 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-let-7 minigene
Vector Backbone Description: Backbone Marker:Carl Thummel Lab; Backbone Size:10200; Vector Backbone:pCaSpeR4-tubulin promoter; Vector Types:Drosophila P-element vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.
Proper citation: RRID:Addgene_17769 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-psm (gagctcACTATACAACCTACAAGCACAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17773 Copy
Species: Drosophila melanogaster
Genetic Insert: cg11094 utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Proper citation: RRID:Addgene_17777 Copy
Species: Drosophila melanogaster
Genetic Insert: cg1849 utr fragment mut
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Proper citation: RRID:Addgene_17780 Copy
Species: Drosophila melanogaster
Genetic Insert: cg5166a/c utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Proper citation: RRID:Addgene_17781 Copy
Species: Drosophila melanogaster
Genetic Insert: cg6551 utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Proper citation: RRID:Addgene_17783 Copy
Species: Drosophila melanogaster
Genetic Insert: cg6551 utr fragment mut
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Proper citation: RRID:Addgene_17784 Copy
Species: Drosophila melanogaster
Genetic Insert: R70B04
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165792 Copy
Species: Drosophila melanogaster
Genetic Insert: Vasa promoter
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165783 Copy
Species: Drosophila melanogaster
Genetic Insert: AlfaTub84b
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165781 Copy
Species: Drosophila melanogaster
Genetic Insert: Hsp70b
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165785 Copy
Species: Drosophila melanogaster
Genetic Insert: Hsp70b-TACT
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165780 Copy
Species: Drosophila melanogaster
Genetic Insert: 3x3-Hsp70b
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165773 Copy
Species: Drosophila melanogaster
Genetic Insert: dSCP
Vector Backbone Description: Backbone Size:2790; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Mini-Prom
Proper citation: RRID:Addgene_165776 Copy
Species: Drosophila melanogaster
Genetic Insert: Actin5c
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165777 Copy
Species: Drosophila melanogaster
Genetic Insert: 3xP3
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165779 Copy
Species: Drosophila melanogaster
Genetic Insert: calx2
Vector Backbone Description: Backbone Size:8780; Vector Backbone:pCaspeRhs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15694324
Proper citation: RRID:Addgene_1741 Copy
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