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Species: Caenorhabditis elegans
Genetic Insert: cha-1
Vector Backbone Description: Vector Backbone:pPD114.108; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Proper citation: RRID:Addgene_78191 Copy
Species: Caenorhabditis elegans
Genetic Insert: cha-1
Vector Backbone Description: Vector Backbone:pPD114.108; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Proper citation: RRID:Addgene_78193 Copy
Species: Caenorhabditis elegans
Genetic Insert: fil-2
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19416714
Proper citation: RRID:Addgene_78188 Copy
Species: Caenorhabditis elegans
Genetic Insert: vab-1
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pSL1190; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9506518
Proper citation: RRID:Addgene_128414 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Psnt-1 | histamine | rpl-3 UTR]
Vector Backbone Description: Backbone Marker:ThermoFischer; Backbone Size:4107; Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33437931
Comments: Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
Proper citation: RRID:Addgene_161515 Copy
Species: Caenorhabditis elegans
Genetic Insert: Caenorhabditis elegans SLOwpoke potassium channel
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pOX; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10903569
Proper citation: RRID:Addgene_16202 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tandem pore domain K+ channel
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pBSc; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8938713
Proper citation: RRID:Addgene_16212 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans iPGM bio-6His
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5446; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33812994
Comments: See also van Neer et al. (2022) "Serum-Stable and Selective Backbone-N-Methylated Cyclic Peptides That Inhibit Prokaryotic Glycolytic Mutases" ACS Chem Biol. PMID 35904259; doi: https://doi.org/10.1021/acschembio.2c00403.
Proper citation: RRID:Addgene_162559 Copy
Species: Caenorhabditis elegans
Genetic Insert: mScarlet^SEC (Lox511I)^::3xMyc
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.12.090217v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_163092 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.12.090217v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_163094 Copy
Species: Caenorhabditis elegans
Genetic Insert: mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.12.090217v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_163093 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.12.090217v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_163096 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.12.090217v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_163095 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_28091 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPB
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26560 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPA
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26559 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS.
Loop mutants have also all been cloned into this backbone for in vitro translation.
Proper citation: RRID:Addgene_26558 Copy
Species: Caenorhabditis elegans
Genetic Insert: dcap-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26938 Copy
Species: Caenorhabditis elegans
Genetic Insert: lag-2 promoter
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid #1497); Backbone Size:4492; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7568229
Comments: Quick confirmation: BamHI should result in two fragments: ~3kb and 4.5kb.
This is a lag-2 promoter::GFP fusion. Strong GFP in live worms. 3kb BamHI fragment of pJK520 is the complete 3kb 5' region of lag-2.
Proper citation: RRID:Addgene_26814 Copy
Species: Caenorhabditis elegans
Genetic Insert: gamma-tubulin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Proper citation: RRID:Addgene_26948 Copy
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