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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pLSSmKate2-N1
 
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1+ mentions
RRID:Addgene_31867 LSS-mKate2 Kanamycin PMID:20212155 Backbone Marker:Clontech; Backbone Size:3998; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin K69Y/P131T/S148G/M167D/T183S/M196V 2026-09-19 02:17:03 2
pLSSmKate2-C1
 
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RRID:Addgene_31869 LSS-mKate2 Kanamycin PMID:20212155 Backbone Marker:Clontech; Backbone Size:3983; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin K69Y/P131T/S148G/M167D/T183S/M196V compared to mKate but numbering is relative to EGFP. 2026-09-19 02:17:03 1
pLSSmKate1-C1
 
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RRID:Addgene_31868 LSS-mKate1 Kanamycin PMID:20212155 Backbone Marker:Clontech; Backbone Size:3983; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin K69Y/P131T/S148G/M167E/T183S/M196V compared to mKate but numbering is relative to EGFP. 2026-09-19 02:17:03 0
Khc73(1-547)-GFP
 
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RRID:Addgene_31749 Khc73 Drosophila melanogaster Ampicillin PMID:21169635 The construct contains V308M and H383R mutations in Khc73 relative to GenBank reference sequences and is as used in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pMTWG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Contains only aa 1-547 2026-09-19 02:17:02 0
pSicoR-Ef1a-mCh-Puro-Luci
 
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RRID:Addgene_31850 Luciferase RNAi Ampicillin PMID:20498046 This vector can be used as an shRNA control. The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α and adding an in-frame T2A-puromycin-resistance gene cassette following mCherry. A single RNA molecule is generated for mCherry-T2A-puromycin and two polypeptides are produced by the T2A ribosomal skip motif. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR. Backbone Marker:Addgene plasmid #31845; Backbone Size:8181; Vector Backbone:pSicoR-Ef1a-mCH-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin 2026-09-19 02:17:03 0
pSC301
 
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RRID:Addgene_31851 GFP (including ATG start codon) Hygromycin PMID:11250077 Plasmid can also be grown in mycobacteria. Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-19 02:17:03 3
pALACE
 
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RRID:Addgene_31853 Hygromycin PMID:12754249 Plasmid can also be grown in mycobacteria. Also see PMID 15342574 for additional reference. Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional. Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:8221; Vector Backbone:pACE; Vector Types:Bacterial Expression, Escherichia coli-Mycobacterium shuttle plasmid; Bacterial Resistance:Hygromycin 2026-09-19 02:17:03 0
pShuttle-CMV-iRFP
 
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1+ mentions
RRID:Addgene_31856 iRFP Rhodopseudomonas palustris Kanamycin PMID:21765402 For additional protocols on using iRFP in other tissue types please review: Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22213541/ For more information on in vivo imaging, please see: https://www.addgene.org/fluorescent_proteins/in_vivo/ Backbone Marker:Stratagene; Backbone Size:7384; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2 2026-09-19 02:17:03 8
psiCHECK-2-nMyc-wt_3'UTR
 
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RRID:Addgene_31858 nMyc wild-type 3'UTR Mus musculus Ampicillin PMID:20054295 Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-19 02:17:03 0
piRFP
 
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10+ mentions
RRID:Addgene_31857 iRFP Rhodopseudomonas palustris Kanamycin PMID:21765402 piRFP was derived from pEGFP-C1, whereby iRFP was inserted in place of EGFP. The iRFP in this plasmid has a stop codon at its end making it unsuitable for generating fusions. This plasmid can be used for cytoplasmic expression of iRFP or as a source of the iRFP gene. For additional protocols on using iRFP in other tissue types please review: Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22213541/ For more information on in vivo imaging, please see: https://www.addgene.org/fluorescent_proteins/in_vivo/ Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2 2026-09-19 02:17:03 43
psiCHECK-2-nMyc-m1_3'UTR
 
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RRID:Addgene_31859 nMyc mutant-1 3'UTR Mus musculus Ampicillin PMID:20054295 Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin first Let-7c Seed changed from TACCTC to TAACGC 2026-09-19 02:17:03 0
pcDNA5FRT/TO-p97-RG-mycStrep
 
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RRID:Addgene_31840 p97 Rattus norvegicus Ampicillin PMID:21822278 Contains NMHTG linker between myc and Strep tags. The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone. Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin R95G 2026-09-19 02:17:03 0
pSicoR-Ef1a-mCh-Puro
 
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10+ mentions
RRID:Addgene_31845 Ampicillin PMID:20498046 The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α and adding an in-frame T2A-puromycin-resistance gene cassette following mCherry. A single RNA molecule is generated for mCherry-T2A-puromycin and two polypeptides are produced by the T2A ribosomal skip motif. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR. Backbone Marker:Addgene plasmid #21907; Backbone Size:8141; Vector Backbone:pSicoR-mCH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin 2026-09-19 02:17:03 19
pSicoR-Ef1a-mCh
 
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10+ mentions
RRID:Addgene_31847 Ampicillin PMID:20498046 The pSicoR-Ef1α-mCh lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR. Backbone Marker:Addgene plasmid #21907; Backbone Size:7484; Vector Backbone:pSicoR-mCH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin 2026-09-19 02:17:03 10
pENTR-myr-AKT-HA
 
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1+ mentions
RRID:Addgene_31790 Akt1 Mus musculus Kanamycin PMID:21147110 Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-09-19 02:17:02 1
pSRP-mTAZ
 
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1+ mentions
RRID:Addgene_31795 TAZ Mus musculus Ampicillin PMID:16099986 Backbone Marker:Drs. S Lessnick and T. Golub (Dana-Farber Cancer Institute); Vector Backbone:pSRP; Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin 2026-09-19 02:17:02 4
pT3TS
 
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1+ mentions
RRID:Addgene_31830 Ampicillin PMID:9891358 Backbone Size:3216; Vector Backbone:pT3TS; Vector Types:; Bacterial Resistance:Ampicillin 2026-09-19 02:17:02 4
pcDNA5FRT/TO-p97-RH-mycStrep
 
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RRID:Addgene_31839 p97 Rattus norvegicus Ampicillin PMID:21822278 Contains NMHTG linker between myc and Strep tags. The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone. Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin R155H 2026-09-19 02:17:03 0
pCDF1-GRM3 (G561E)
 
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RRID:Addgene_31799 GRM3 Homo sapiens Ampicillin PMID:21946352 Backbone Marker:SBI; Backbone Size:6600; Vector Backbone:pCDF1- MCS2-EF1-puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Changed glycine 561 to glutamic acid 2026-09-19 02:17:02 0
pVSV eGFP RABV-G
 
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RRID:Addgene_31833 VSV genome Ampicillin PMID:21825165 This plasmid could potentially be used to generate BL2 virus. It is the responsibility of the recipient scientist to ensure that they are in compliance with their institutions’ biosafety requirements. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:02 3

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