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Species: Saccharomyces cerevisiae
Genetic Insert: Nsr1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63769 Copy
Species: Drosophila melanogaster
Genetic Insert: SP-dCas9-VPR
Vector Backbone Description: Vector Backbone:pAWG; Vector Types:Insect Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Please note that this plasmid contains a stop codon between dCas9-VPR and eGFP and does NOT produce a GFP fusion protein.
Cas9 contains D839A and N863A mutations. Depositor states that these mutations should not affect function.
Proper citation: RRID:Addgene_63802 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63803 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63804 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nog2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63764 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nop2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63766 Copy
Species: Homo sapiens
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:PB-TRE; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Piggybac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Proper citation: RRID:Addgene_63800 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63806 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mak16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63762 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tma16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63779 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63812 Copy
Species: Other
Genetic Insert: PhlF and LacI
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63815 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63810 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rrp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63773 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Puf6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63770 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63823 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63824 Copy
Species: E. coli cloning vector
Genetic Insert: eCitrine-Strep-tag II followed by URA3 selection marker
Vector Backbone Description: Backbone Marker:Mark A. Sheff and Kurt S. Thorn; Backbone Size:4900; Vector Backbone:pKT175; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.
Proper citation: RRID:Addgene_63787 Copy
Species: Synthetic
Genetic Insert: eVenus-Strep-tag II followed by URA3
Vector Backbone Description: Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: The sequence of the eVenus gene has been optimized for S. cerevisiae and generated by gene synthesis. The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.
Proper citation: RRID:Addgene_63788 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63821 Copy
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