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Species: Homo sapiens
Genetic Insert: Mini-intronic-plasmid intron
Vector Backbone Description: Backbone Size:7608; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:RNA-OUT ( 6% sucrose)
Defining Citation: PMID:25628230
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.
Proper citation: RRID:Addgene_63727 Copy
Species: Streptococcus pneumoniae
Genetic Insert: acpS
Vector Backbone Description: Backbone Marker:Novagen ; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63687 Copy
Species: Mus musculus
Genetic Insert: BLBP
Vector Backbone Description: Backbone Size:1700; Vector Backbone:pCAG-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23345401
Proper citation: RRID:Addgene_63721 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5731; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_63723 Copy
Species: Homo sapiens
Genetic Insert: Lin28
Vector Backbone Description: Vector Backbone:no backbone--uses MiP intron for replication in bacteria; Vector Types:Mammalian Expression; Bacterial Resistance:RNA-OUT ( 6% sucrose)
Defining Citation: PMID:25628230
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.
Proper citation: RRID:Addgene_63729 Copy
Species: Staphylococcus aureus
Genetic Insert: ACP
Vector Backbone Description: Backbone Marker:Novagen ; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63686 Copy
Vector Backbone Description: Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_63681 Copy
Genetic Insert: FLIPQTV3.0 sensor, 2mM affinity
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22723868
Proper citation: RRID:Addgene_63731 Copy
Vector Backbone Description: Backbone Size:9231; Vector Backbone:pSN40; Vector Types:C. albicans bait plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20719741
Comments: Note--the ccdB gene contained is inactive as it is missing the start codon so no ccd survival strain is necessary.
Proper citation: RRID:Addgene_63734 Copy
Species: V. cholerae
Genetic Insert: nanH
Vector Backbone Description: Backbone Marker:Franklin lab; Vector Backbone:pCVD315; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2832365
Comments: See also Galen et. al. 1990. Advances in Research on Cholera and related Diarrheas, 7, eds., R.B. Sack, Y. Zinnaka p143-153 KTK Scientific Publishers, Tokyo
Proper citation: RRID:Addgene_63739 Copy
Species: Stickleback (G. aculeatus)
Genetic Insert: 3.2 kb marine stickleback armor plate enhancer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4890; Vector Backbone:pTA-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25629660
Proper citation: RRID:Addgene_63696 Copy
Species: Other
Genetic Insert: ctxA
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: Kaper, J. B., J. G. Morris, Jr., and M. Nishibuchi. 1988. DNA probes for pathogenic Vibrio species, p. 65-77. In F. C. Tenover (ed.), DNA probes for infectious disease. CRC Press, Inc., Boca, Raton, Fla.
Proper citation: RRID:Addgene_63690 Copy
Species: T7 bacteriophage
Genetic Insert: K1F IKR RNAP
Vector Backbone Description: Vector Backbone:pQE; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25279711
Proper citation: RRID:Addgene_63626 Copy
Species: T7 bacteriophage
Genetic Insert: CTGA-R13-AKSIRV RNAP
Vector Backbone Description: Vector Backbone:pQE; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25279711
Proper citation: RRID:Addgene_63627 Copy
Species: Drosophila melanogaster
Genetic Insert: immune deficiency
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: *Note: Addgene's quality control sequencing finds two amino acid residue substitutions, L47M and H212Y, in the Drosophila IMD insert. These residue changes are not thought to affect plasmid function, and the protein has been shown to activate the Imd pathway as expected.
Proper citation: RRID:Addgene_63748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dbp9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63743 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Drs1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25877921
Proper citation: RRID:Addgene_63744 Copy
Species: Other
Genetic Insert: LT gene probe
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2254425
Comments: The LT probe is a 1-kilobase BamHI fragment derived from pWD299. The HincII fragment was removed from pWD299, BamHI linkers were attached, and this fragment was cloned into pACYC184
Proper citation: RRID:Addgene_63740 Copy
Species: Enteropathogenic Escherichia coli
Genetic Insert: eae
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2172966
Proper citation: RRID:Addgene_63759 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pAG414GPD; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 contains D839A and N863A mutations relative to reference sequence (WP_010922251.1). Depositor states that these mutations do not affect function.
Proper citation: RRID:Addgene_63801 Copy
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