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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pH_35S/mEGFP
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_135321 mEGFP Synthetic Spectinomycin PMID:32096870 Please visit https://www.biorxiv.org/content/10.1101/852020v1 for bioRxiv preprint. Backbone Size:9717; Vector Backbone:pN_35S; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin Addgene 2026-09-26 02:23:47 2
pET26b_Nb.AT110
 
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RRID:Addgene_135480 Nanobody AT110 Synthetic Kanamycin PMID:30639100 Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Addgene 2026-09-26 02:23:49 0
pET26b_Nb.AT110.i1
 
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RRID:Addgene_135481 Nanobody AT110.i1 Synthetic Kanamycin PMID:30639100 Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Addgene 2026-09-26 02:23:49 0
pCAG_Xph15-eGFP-CCR5TC
 
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RRID:Addgene_135528 Xph15 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:7120; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 0
pCAG_Xph20-eGFP-CCR5TC
 
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RRID:Addgene_135530 Xph20 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:7120; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 0
pCAG_Xph20-mRuby2-CCR5TC
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_135531 Xph20 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 1
pCAG_Xph20-mEos3.2-CCR5TC
 
Resource Report
Resource Website
RRID:Addgene_135532 Xph20 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 0
pCAG_Xph20-mNeonGreen-CCR5TC
 
Resource Report
Resource Website
RRID:Addgene_135534 Xph20 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 0
pCAG_Xph20-SNAPf-CCR5TC
 
Resource Report
Resource Website
RRID:Addgene_135537 Xph20 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 0
pCAG_Xph20-GCaMP7f
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_135539 Xph20 Synthetic Ampicillin PMID:38167295 Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint. Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6211; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:49 2
Gal:mCherry-CRY2-uTEV1Δ(220-242)
 
Resource Report
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RRID:Addgene_135452 mCherry-CRY2-TEV1Δ(220-242) Synthetic Ampicillin PMID:31819267 Backbone Marker:Steven B. Haase; Backbone Size:5807; Vector Backbone:pRSII 413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:48 0
Gal:mCherry-uTEV3
 
Resource Report
Resource Website
RRID:Addgene_135453 mCherry-uTEv3 Synthetic Ampicillin PMID:31819267 Backbone Marker:Steven B. Haase; Backbone Size:5807; Vector Backbone:pRSII 413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin S219V mutation improves stability , I138T/S153N/T180A mutations improve catalytic activity Addgene 2026-09-26 02:23:48 0
UAS-mCherry
 
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RRID:Addgene_135457 mCherry Synthetic Ampicillin PMID:31819267 Backbone Marker:Stratagene; Backbone Size:4263; Vector Backbone:AAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:48 0
CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
 
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RRID:Addgene_135458 β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5 Synthetic Ampicillin PMID:31819267 Hygromycin selection gene was deleted Backbone Size:6775; Vector Backbone:pLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:48 0
CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4
 
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RRID:Addgene_135460 CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape Synthetic Ampicillin PMID:31819267 Backbone Marker:Stratagene; Backbone Size:3805; Vector Backbone:AAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:48 0
Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA
 
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RRID:Addgene_135462 NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA Synthetic Ampicillin PMID:31819267 Reporter gene associated with this construct is Addgene plasmid # 92202 published in: Nat. Biotechnol. 35, 864–871 (2017). Backbone Marker:Stratagene; Backbone Size:3663; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:48 0
His6-MBP-uTEV3
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_135464 His6-MBP-uTEV3 Synthetic Ampicillin PMID:31819267 Backbone Marker:John Cronan; Backbone Size:4857; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin S219V mutation (improves stability) , I138T/S153N/T180A mutations improve activity. Last 6 amino acids from TEV were deleted (237-242) Addgene 2026-09-26 02:23:48 1
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
 
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RRID:Addgene_135465 STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16 Synthetic Ampicillin PMID:31819267 For integration in yeast, linearize plasmid with AscI. The reporter genes associated with this construct are #58432, #58433 and #58434 published in: Nucleic Acids Res. 42, e130–e130 (2014) Backbone Marker:Robert Gnuegge; Backbone Size:5660; Vector Backbone:pRG205; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:23:48 0
pQlox71R
 
Resource Report
Resource Website
RRID:Addgene_135656 Intron containing lox site Synthetic Erythromycin PMID:31826971 Use in combination with pQlox66F (addgene 135657) or pQlox 66R (addgene 135658), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin Addgene 2026-09-26 02:23:50 0
pQadd2
 
Resource Report
Resource Website
RRID:Addgene_135662 Lox511/66 and LoxFas/71 Synthetic Erythromycin PMID:31826971 Cargo can be inserted between the lox sites using the SpeI and XhoI sites. Vector Backbone:pAT19; Vector Types:Bacterial Expression, Clostridial vector, pAMβ1 origin; Bacterial Resistance:Erythromycin Addgene 2026-09-26 02:23:50 0

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