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Species: Synthetic
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Size:9717; Vector Backbone:pN_35S; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32096870
Comments: Please visit https://www.biorxiv.org/content/10.1101/852020v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_135321 Copy
Species: Synthetic
Genetic Insert: Nanobody AT110
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30639100
Proper citation: RRID:Addgene_135480 Copy
Species: Synthetic
Genetic Insert: Nanobody AT110.i1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET26b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30639100
Proper citation: RRID:Addgene_135481 Copy
Species: Synthetic
Genetic Insert: Xph15
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:7120; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135528 Copy
Species: Synthetic
Genetic Insert: Xph20
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:7120; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135530 Copy
Species: Synthetic
Genetic Insert: Xph20
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135531 Copy
Species: Synthetic
Genetic Insert: Xph20
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135532 Copy
Species: Synthetic
Genetic Insert: Xph20
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135534 Copy
Species: Synthetic
Genetic Insert: Xph20
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6678; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135537 Copy
Species: Synthetic
Genetic Insert: Xph20
Vector Backbone Description: Backbone Marker:Don B. Arnold lab (USC); Backbone Size:6211; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38167295
Comments: Please visit https://doi.org/10.1101/2021.04.07.438431 for bioRxiv preprint.
Proper citation: RRID:Addgene_135539 Copy
Species: Synthetic
Genetic Insert: mCherry-CRY2-TEV1Δ(220-242)
Vector Backbone Description: Backbone Marker:Steven B. Haase; Backbone Size:5807; Vector Backbone:pRSII 413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Proper citation: RRID:Addgene_135452 Copy
Species: Synthetic
Genetic Insert: mCherry-uTEv3
Vector Backbone Description: Backbone Marker:Steven B. Haase; Backbone Size:5807; Vector Backbone:pRSII 413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Proper citation: RRID:Addgene_135453 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4263; Vector Backbone:AAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Proper citation: RRID:Addgene_135457 Copy
Species: Synthetic
Genetic Insert: β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
Vector Backbone Description: Backbone Size:6775; Vector Backbone:pLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Comments: Hygromycin selection gene was deleted
Proper citation: RRID:Addgene_135458 Copy
Species: Synthetic
Genetic Insert: CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3805; Vector Backbone:AAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Proper citation: RRID:Addgene_135460 Copy
Species: Synthetic
Genetic Insert: NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3663; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Comments: Reporter gene associated with this construct is Addgene plasmid # 92202 published in: Nat. Biotechnol. 35, 864–871 (2017).
Proper citation: RRID:Addgene_135462 Copy
Species: Synthetic
Genetic Insert: His6-MBP-uTEV3
Vector Backbone Description: Backbone Marker:John Cronan; Backbone Size:4857; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Proper citation: RRID:Addgene_135464 Copy
Species: Synthetic
Genetic Insert: STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:5660; Vector Backbone:pRG205; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31819267
Comments: For integration in yeast, linearize plasmid with AscI.
The reporter genes associated with this construct are #58432, #58433 and #58434 published in: Nucleic Acids Res. 42, e130–e130 (2014)
Proper citation: RRID:Addgene_135465 Copy
Species: Synthetic
Genetic Insert: Intron containing lox site
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin
Defining Citation: PMID:31826971
Comments: Use in combination with pQlox66F (addgene 135657) or pQlox 66R (addgene 135658), then pQcre1 (addgene 135659).
Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites.
Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details.
Intron redesign links
http://www.clostron.com/clostron1.php
http://www.targetrons.com/targetron_pLtrB.php
Reference for intron redesign
Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464.
Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439.
Proper citation: RRID:Addgene_135656 Copy
Species: Synthetic
Genetic Insert: Lox511/66 and LoxFas/71
Vector Backbone Description: Vector Backbone:pAT19; Vector Types:Bacterial Expression, Clostridial vector, pAMβ1 origin; Bacterial Resistance:Erythromycin
Defining Citation: PMID:31826971
Comments: Cargo can be inserted between the lox sites using the SpeI and XhoI sites.
Proper citation: RRID:Addgene_135662 Copy
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