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Species: Synthetic
Genetic Insert: Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
Vector Backbone Description: Backbone Size:1960; Vector Backbone:pUC; Vector Types:Mammalian Expression, Cre/Lox, FRT/Flpe recombination mediated insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26492136
Proper citation: RRID:Addgene_63577 Copy
Species: Homo sapiens
Genetic Insert: USP7
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21981925
Comments: Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_63573 Copy
Species: Mus musculus
Genetic Insert: Col1A-frt-hygro-pA
Vector Backbone Description: Backbone Size:2888; Vector Backbone:pBluescriptII SK; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, FRT/Flpe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26492136
Comments: The cassette in between the homologous recombination arms is the same as in AddGene Plasmid #20730.
The homologous recombination arms are derived from mouse strain FVB.
The Hygromycin gene in this construct does not have an ATG start codon nor is it expressed. Hygromycin can not be used for selection when this construct is used in a targeting experiment in mouse strain FVB derived ES cells.
The construct can be linearized with AscI, FseI or XhoI
Proper citation: RRID:Addgene_63575 Copy
Species: Homo sapiens
Genetic Insert: UBA1
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15723079
Proper citation: RRID:Addgene_63571 Copy
Species: Homo sapiens
Genetic Insert: UBE2-25K
Vector Backbone Description: Vector Backbone:pGEX4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15723079
Proper citation: RRID:Addgene_63572 Copy
Species: T7 bacteriophage
Genetic Insert: CTGA-R13-AKSRV RNAP
Vector Backbone Description: Vector Backbone:pQE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25279711
Proper citation: RRID:Addgene_63622 Copy
Species: T7 bacteriophage
Genetic Insert: T3 RRVH RNAP
Vector Backbone Description: Vector Backbone:pQE; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25279711
Proper citation: RRID:Addgene_63623 Copy
Species: Synthetic
Genetic Insert: HD HD NG
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63509 Copy
Genetic Insert: VfAU1-LOV
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63632 Copy
Species: Drosophila melanogaster
Genetic Insert: TGF-β activated kinase 1
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63753 Copy
Species: Other
Genetic Insert: EAggEC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2189007
Comments: DNA probe for the identification of enteroaggregative E. coli
Proper citation: RRID:Addgene_63755 Copy
Species: Drosophila melanogaster
Genetic Insert: Relish
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63750 Copy
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Features list:
USER cassette 1..14
T CYC1 20..190
loxP 257..316
T URA3 317..439
Degradation tag 440..490
K. l. LEU2 491..1576
P K. l. LEU2 1577..2606
loxP 2623..2656
Consensus TY1Cons2 3' 2796..3001
pUC ori 3775..4418
AmpR 4521..5379
Consensus TY1Cons2 5' 5823..6054
T ADH1 6081..6275
Proper citation: RRID:Addgene_63643 Copy
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
USER cassette 1..14
T CYC1 20..190
loxP 257..316
T URA3 317..439
Degradation tag 440..490
K. l. LEU2 491..1576
P K. l. LEU2 1577..2606
loxP 2623..2656
Consensus TY3 3' 2796..2996
pUC ori 3777..4420
AmpR 4523..5381
Consensus TY3 5' 5825..6058
T ADH1 6085..6279
Proper citation: RRID:Addgene_63645 Copy
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
USER cassette 1..14
T CYC1 20..209
loxP 257..316
T URA3 317..439
Degradation tag 440..490
K.l. URA3 491..1291
P K.l. URA3 1292..1790
loxP 1807..1840
Consensus TY4 3' 1995..2180
pUC ori 2978..3621
AmpR 3724..4582
Consensus TY4 5' 5063..5247
T ADH1 5292..5486
Proper citation: RRID:Addgene_63641 Copy
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
USER cassette 1..14
T CYC1 20..190
loxP 257..316
T K.l. URA3 317..439
Degradation tag 440..490
K. l. LEU2 491..1576
P K. l. LEU2 1577..2606
loxP 2623..2656
Consensus TY1Cons1 3' 2796..3003
pUC ori 3776..4419
AmpR 4522..5380
Consensus TY1Cons1 5' 5824..6057
T ADH1 6084..6278
Proper citation: RRID:Addgene_63642 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l. LEU2 1563..2592
loxP 2609..2642
Consensus TY4 3' 2797..2982
pUC ori 3780..4423
AmpR 4526..5384
Consensus TY4 5' 5865..6049
T ADH1 6094..6288
P TEF1 6307..6726
GFP 6736..7452
Proper citation: RRID:Addgene_63659 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l. LEU2 1563..2592
loxP 2609..2642
Consensus Ty1Cons1 3' 2782..2989
pUC ori 3762..4405
AmpR 4508..5366
Consensus Ty1Cons1 5' 5810..6043
T ADH1 6070..6264
P TEF1 6283..6702
GFP 6712..7428
Proper citation: RRID:Addgene_63655 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l LEU2 1563..2592
loxP 2609..2642
Consensus Ty1Cons2 3' 2782..2987
pUC ori 3761..4404
AmpR 4507..5365
Consensus Ty1Cons2 5' 5809..6040
T ADH1 6067..6261
P TEF1 6280..6699
GFP 6709..7425
Proper citation: RRID:Addgene_63656 Copy
Species: Homo sapiens
Genetic Insert: Mps1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7305; Vector Backbone:pRetrox; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25197064
Proper citation: RRID:Addgene_63702 Copy
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