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Species: Synthetic
Genetic Insert: YE1-BE3-FNLS
Vector Backbone Description: Vector Backbone:unspecified; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32424272
Comments: INSERT: YE1-BE3-FNLS driven by CMV promoter.
Proper citation: RRID:Addgene_154005 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: S
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Comments: Kim et al. 2020 can be accessed at https://doi.org/10.1016/j.cell.2020.04.011
Proper citation: RRID:Addgene_153952 Copy
Species: Synthetic
Genetic Insert: CasRx
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32272060
Comments: INSERT: CasRx driven by GFAP promoter.
Proper citation: RRID:Addgene_154000 Copy
Species: Synthetic
Genetic Insert: SgRNA expression system
Vector Backbone Description: Vector Backbone:PB; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32185621
Comments: INSERT: mCherry driven by EF1a promoter.
INSERT: DR-SgRNA driven by U6 promoter.
Proper citation: RRID:Addgene_154002 Copy
Species: Synthetic
Genetic Insert: CasRx
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32185621
Comments: INSERT: CasRx driven by EFS promoter.
INSERT: DR-SgRNA_LacZ driven by U6 promoter.
Proper citation: RRID:Addgene_154003 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5788; Vector Backbone:pIREShyg2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31995758
Proper citation: RRID:Addgene_153966 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:pUC57 mini; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32559415
Proper citation: RRID:Addgene_154019 Copy
Species: Homo sapiens
Genetic Insert: IRAK1
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMV6-Entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32161170
Proper citation: RRID:Addgene_153968 Copy
Species: Homo sapiens
Genetic Insert: Axin1
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6716; Vector Backbone:pMBP-MG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553184
Proper citation: RRID:Addgene_154012 Copy
Species: Synthetic
Genetic Insert: 5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
Vector Backbone Description: Backbone Size:5330; Vector Backbone:pBABEneo; Vector Types:Mammalian Expression, Retroviral, A reporter plasmid detecting DNA recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31995758
Proper citation: RRID:Addgene_153960 Copy
Species: Homo sapiens
Genetic Insert: GSK3B
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6716; Vector Backbone:pMBP-MG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553184
Proper citation: RRID:Addgene_154010 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway-compatible Entry vector, with mutagenized insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154029 Copy
Species: P. tricornutum
Genetic Insert: Terminator 45582
Vector Backbone Description: Vector Backbone:pL0RlacZ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_154037 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Diatom cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: tet resistance cassette removed; ccdb resistance with att sites added; selectable marker added for centric diatoms
Proper citation: RRID:Addgene_154033 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Diatom cloning; Bacterial Resistance:Ampicillin and Tetracycline
Comments: selectable marker added for centric diatoms
Proper citation: RRID:Addgene_154034 Copy
Species: Homo sapiens
Genetic Insert: MED17
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5700; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14576168
Proper citation: RRID:Addgene_15412 Copy
Species: P. tricornutum
Genetic Insert: Promoter SVP
Vector Backbone Description: Vector Backbone:pL0RlacZ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_154040 Copy
Species: P. tricornutum
Genetic Insert: Terminator SVP
Vector Backbone Description: Vector Backbone:pL0RlacZ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_154041 Copy
Species: P. tricornutum
Genetic Insert: Terminator Pbt
Vector Backbone Description: Vector Backbone:pL0RlacZ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_154043 Copy
Species: E. coli
Genetic Insert: beta-glucoronidase (E. coli)
Vector Backbone Description: Backbone Marker:Engineering Nitrogen Symbiosis for Africa (ENSA); Backbone Size:2503; Vector Backbone:EC10161; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33072173
Proper citation: RRID:Addgene_154059 Copy
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