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Species: Synthetic
Genetic Insert: NG NI NG
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63535 Copy
Species: Synthetic
Genetic Insert: NN NN NI
Vector Backbone Description: Backbone Size:3008; Vector Backbone:FusX4; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63549 Copy
Species: Synthetic
Genetic Insert: NG NG NI
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63544 Copy
Species: Synthetic
Genetic Insert: NG NG NN
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63546 Copy
Species: Synthetic
Genetic Insert: NG NN NN
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63542 Copy
Species: Synthetic
Genetic Insert: NG NN NG
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63543 Copy
Species: Leishmania
Genetic Insert: guide sequence
Vector Backbone Description: Vector Backbone:pSPneo; Vector Types:Leishmania Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199327
Comments: The guide sequence will be cloned into the two Bbs I sites ( See attached map). This plasmid contains secondary structure and needs to be sequenced with a modified protocol.
Proper citation: RRID:Addgene_63556 Copy
Species: Leishmania
Genetic Insert: guide sequence
Vector Backbone Description: Vector Backbone:pSPneo; Vector Types:Leishmania Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199327
Comments: The guide sequence will be cloned into the two Bbs I sites (See attached map). This plasmid contains secondary structure and needs to be sequenced with a modified protocol.
Proper citation: RRID:Addgene_63557 Copy
Species: Synthetic
Genetic Insert: NG NG NG
Vector Backbone Description: Backbone Size:3008; Vector Backbone:FusX4; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63552 Copy
Species: Synthetic
Genetic Insert: NG NG HD
Vector Backbone Description: Backbone Size:3008; Vector Backbone:FusX4; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63550 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63603 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63604 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63606 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980979
Proper citation: RRID:Addgene_63566 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980979
Proper citation: RRID:Addgene_63567 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980979
Proper citation: RRID:Addgene_63568 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63601 Copy
Species: Mus musculus
Genetic Insert: Bicaudal D protein
Vector Backbone Description: Backbone Size:7041; Vector Backbone:pBa-eGFP-FKBP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_63569 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63602 Copy
Vector Backbone Description: Backbone Marker:Mullins, E.D., Kang, S., 2001. Transformation: a tool for studying fungal pathogens of plants. Cell. Mol. Life Sci. 58, 2043–205; Backbone Size:7483; Vector Backbone:pDHt; Vector Types:fungal expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15893252
Proper citation: RRID:Addgene_63615 Copy
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