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Species: Saccharomyces cerevisiae
Genetic Insert: ATG18
Vector Backbone Description: Backbone Size:6112; Vector Backbone:Ycplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Comments: ATG18 was cloned with 300bp upstream and downstream of the gene. The myc tag was added later by mutagenesis.
Proper citation: RRID:Addgene_42530 Copy
Species: cyanobacteria
Genetic Insert: KaiC CII cat-/EE (E318Q; S431E; T432E)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2200; Vector Backbone:prset-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23277568
Proper citation: RRID:Addgene_42498 Copy
Species: Arabidopsis thaliana
Genetic Insert: FKF1 (N-LOV, G128D mutant) fused with VP16
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801976
Proper citation: RRID:Addgene_42499 Copy
Species: Homo sapiens
Genetic Insert: UPASE2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2XRF/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsv2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHIS2 parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Proper citation: RRID:Addgene_42524 Copy
Species: Homo sapiens
Genetic Insert: NISCHA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3P0C/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42484 Copy
Species: Homo sapiens
Genetic Insert: PARP14A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3Q71/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42488 Copy
Species: Mus musculus
Genetic Insert: Rosa26 genomic fragment
Vector Backbone Description: Vector Backbone:pF2; Vector Types:Mouse Targeting; Bacterial Resistance:Triclosan
Defining Citation: PMID:23437314
Proper citation: RRID:Addgene_42522 Copy
Species: Homo sapiens
Genetic Insert: G3BP1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3Q90/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42480 Copy
Species: Homo sapiens
Genetic Insert: INPP5EA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2XSW/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42481 Copy
Species: Homo sapiens
Genetic Insert: ERI3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2XRI/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42479 Copy
Species: Mus musculus
Genetic Insert: Pyruvate Kinase M2 Cys358->Ser
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: Changed Cys358 codon to Ser358 by 2-step PCR mutagenesis using pLHCX-FT-PKM2 as template
Proper citation: RRID:Addgene_42513 Copy
Species: Mus musculus
Genetic Insert: Pyruvate Kinase M2 Lys433->Glu
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., & Cantley, L. C. (2008). Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature, 452(7184), 181–186. doi:10.1038/nature06667
Proper citation: RRID:Addgene_42514 Copy
Species: Homo sapiens
Genetic Insert: shRNA-PKM2
Vector Backbone Description: Backbone Marker:The RNAi consortium; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect.
Proper citation: RRID:Addgene_42516 Copy
Species: Homo sapiens
Genetic Insert: NISCHA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3P0C/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42477 Copy
Species: Homo sapiens
Genetic Insert: CPSF7A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3N9U/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42470 Copy
Species: Streptomyces coelicolor A3(2)
Genetic Insert: guanosine ADP-ribosyltransferase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6703; Vector Backbone:pMAL-p2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23212047
Proper citation: RRID:Addgene_42509 Copy
Species: Homo sapiens
Genetic Insert: EBF3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3N50/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42468 Copy
Species: Homo sapiens
Genetic Insert: HSPA9A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3N8E/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42469 Copy
Species: Homo sapiens
Genetic Insert: soluble PACE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5077; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23205847
Proper citation: RRID:Addgene_42503 Copy
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