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Species: Caenorhabditis elegans
Genetic Insert: C. elegans glutamate-gated chloride channel alpha, crystallized construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pFastbac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572436
Proper citation: RRID:Addgene_31488 Copy
Genetic Insert: Delta-selective C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20959447
Proper citation: RRID:Addgene_31526 Copy
Species: Homo sapiens
Genetic Insert: YFP-HA-Protein Kinase D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19581308
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It should work fine with the 12CA5 HA antibody.
Proper citation: RRID:Addgene_31527 Copy
Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2760; Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21631110
Proper citation: RRID:Addgene_31472 Copy
Species: Homo sapiens
Genetic Insert: small fragment nuclease
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31593 Copy
Species: fluorescent proteins
Genetic Insert: eYFP and mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:2850; Vector Backbone:pTRETightBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857679
Comments: mCherry fused 3'UTR composed of SLC6A1, which contains three binding sites for the neuronal miRNA miR-218
Proper citation: RRID:Addgene_31471 Copy
Species: Homo sapiens
Genetic Insert: preimplantation protein 3
Vector Backbone Description: Backbone Size:3460; Vector Backbone:pQStrep2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: Please note that there is a small gap between Addgene's quality control sequence and the reference sequence provided by the depositor. The gap is downstream of the ORF and should not affect function.
Proper citation: RRID:Addgene_31592 Copy
Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:British Biotechnology; Backbone Size:5300; Vector Backbone:GW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21631110
Proper citation: RRID:Addgene_31473 Copy
Genetic Insert: SFFV d14 GFP donor EF1s I-SceI
Vector Backbone Description: Backbone Size:5092; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31476 Copy
Genetic Insert: SFFV d14GFP
Vector Backbone Description: Backbone Size:5516; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31475 Copy
Genetic Insert: SFFV HA NLS Y2 K227M T2A BFP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31478 Copy
Species: Mus musculus
Genetic Insert: miR-196a2
Vector Backbone Description: Backbone Size:0; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21451113
Proper citation: RRID:Addgene_31511 Copy
Genetic Insert: SFFV HA NLS Y2 T2A mTagBFP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31477 Copy
Species: Mus musculus
Genetic Insert: miR-196a1
Vector Backbone Description: Backbone Size:0; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21451113
Proper citation: RRID:Addgene_31510 Copy
Species: Mus musculus
Genetic Insert: miR-196b
Vector Backbone Description: Backbone Size:0; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21451113
Proper citation: RRID:Addgene_31513 Copy
Species: Mus musculus
Genetic Insert: miR-196b
Vector Backbone Description: Backbone Size:0; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21451113
Proper citation: RRID:Addgene_31514 Copy
Genetic Insert: linker
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21722715
Comments: pRS plasmid series originally constructed by Sikorski and Hieter (Sikorski, R.S. and Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122 (1989), pp. 19-27.)
Proper citation: RRID:Addgene_31461 Copy
Genetic Insert: linker
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21722715
Comments: pRS plasmid series originally constructed by Sikorski and Hieter (Sikorski, R.S. and Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122 (1989), pp. 19-27.)
Proper citation: RRID:Addgene_31460 Copy
Species: Homo sapiens
Genetic Insert: syntaxin 6
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31581 Copy
Species: Homo sapiens
Genetic Insert: growth arrest and DNA-damage-inducible, gamma
Vector Backbone Description: Backbone Size:3460; Vector Backbone:pQStrep2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct.
Proper citation: RRID:Addgene_31584 Copy
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