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Species: Homo sapiens
Genetic Insert: CFAP410
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: CFAP410 (NM_001271441) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176948 Copy
Species: Homo sapiens
Genetic Insert: TMEM50B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: TMEM50B (NM_006134) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176940 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: 8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF302069, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177023 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: iridoid oxidase; CYP76A26
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF302066, amplified from cDNA, contains T359S relative to KF302066, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177026 Copy
Species: Nepeta mussinii (aka Nepeta racemosa)
Genetic Insert: major latex protein-like
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: MT108267.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177025 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase; UGT709C2
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF302068, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177027 Copy
Species: Picea abies (Norway spruce)
Genetic Insert: geranyl pyrophosphate synthase; geranyl diphosphate synthase
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: GQ369788, transit peptide removed, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177020 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: AJ251269, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177022 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: geraniol synthase
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: JN882024, transit peptide removed, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177021 Copy
Species: SARS-CoV-2
Genetic Insert: S-protein P1
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177095 Copy
Species: SARS-CoV-2
Genetic Insert: S-protein B.1.617.2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177097 Copy
Species: SARS-CoV-2
Genetic Insert: S-protein 501V2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177096 Copy
Species: Other
Genetic Insert: 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47772 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177047 Copy
Species: Other
Genetic Insert: geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177042 Copy
Species: Other
Genetic Insert: geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177041 Copy
Species: Other
Genetic Insert: geraniol synthase (CrGES) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177044 Copy
Species: Other
Genetic Insert: geraniol synthase (CrGES) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177043 Copy
Species: Streptomyces phage phiC31
Genetic Insert: phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: codon optimized for N. benthamiana expression, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard, derived from GB1496
Proper citation: RRID:Addgene_177035 Copy
Species: Other
Genetic Insert: 2x opattB binding site plus minimal CaMV 35S promoter plus partial TMV 'UTR
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177037 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: secologanin synthase; CYP72C
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF309242.1 or KF415117.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177030 Copy
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