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Species: Synthetic
Genetic Insert: polyhistidine (6x) tag
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54446 Copy
Species: Synthetic
Genetic Insert: Nuclear Export Signal (NES) of heat stable inhibitor (PKI)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54445 Copy
Species: Synthetic
Genetic Insert: Nuclear localization signal (NLS) of SV40 large T antigen
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54443 Copy
Species: Synthetic
Genetic Insert: mOrange without Start codon
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54441 Copy
Species: Synthetic
Genetic Insert: polyhistidine (6x) tag
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54447 Copy
Species: Synthetic
Genetic Insert: destablized GFP
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54454 Copy
Species: Synthetic
Genetic Insert: 16 aa GS linker + superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54452 Copy
Species: Synthetic
Genetic Insert: c-MYC epitope tag (EQKLISEEDL)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54450 Copy
Species: Synthetic
Genetic Insert: hygromycin phosphotranferase (hptII) (pCAMBIA)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54463 Copy
Species: Synthetic
Genetic Insert: superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54461 Copy
Species: Synthetic
Genetic Insert: SRM (sortase-recognition motif)
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:yeast genome targeting; Bacterial Resistance:Ampicillin
Comments: Based on the plasmids from Mark Hochstrasser for epitope tagging in S. cerevisiae:
http://www.addgene.org/browse/article/2501/
sortase sequence = LPETG
full sortase recognition motif = GSLPETGGGRR
Proper citation: RRID:Addgene_54489 Copy
Species: Synthetic
Genetic Insert: SRM (sortase-recognition motif)
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:yeast genome targeting; Bacterial Resistance:Ampicillin
Comments: Based on the plasmids from Mark Hochstrasser for epitope tagging in S. cerevisiae:
http://www.addgene.org/browse/article/2501/
sortase sequence = LPETG
full sortase recognition motif = GSLPETGGGRR
Proper citation: RRID:Addgene_54488 Copy
Species: Synthetic
Genetic Insert: His10
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA
Proper citation: RRID:Addgene_55179 Copy
Species: Synthetic
Genetic Insert: cMyc tag
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA
Proper citation: RRID:Addgene_55183 Copy
Species: Synthetic
Genetic Insert: NI NN NG
Vector Backbone Description: Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63357 Copy
Species: Synthetic
Genetic Insert: NI NG NI
Vector Backbone Description: Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63358 Copy
Species: Synthetic
Genetic Insert: NI HD NG
Vector Backbone Description: Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63353 Copy
Species: Synthetic
Genetic Insert: NI NN NI
Vector Backbone Description: Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63354 Copy
Species: Synthetic
Genetic Insert: NI NN HD
Vector Backbone Description: Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63355 Copy
Species: Synthetic
Genetic Insert: NI NN NN
Vector Backbone Description: Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63356 Copy
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