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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W Resource Report Resource Website 1+ mentions |
RRID:Addgene_32520 | Constitutively active Rheb (S16H) | Rattus norvegicus | Ampicillin | PMID:20062052 | The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis. The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005). | Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | S16H to make constitutively active | 2026-08-01 01:11:42 | 2 |
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μ2-HA-D176A Resource Report Resource Website 1+ mentions |
RRID:Addgene_32754 | μ2 subunit of clathrin adaptor complex AP-2 | Rattus norvegicus | Ampicillin | PMID:10228163 | NB: The HA tag (YPYDVPDYALE) was inserted within the protein between residues 236 and 237. | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D176A | 2026-08-01 01:11:45 | 2 |
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μ2-HA-WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_32752 | μ2 subunit of clathrin adaptor complex AP-2 | Rattus norvegicus | Ampicillin | PMID:10228163 | NB: The HA tag (YPYDVPDYALE) was inserted within the protein, between residues 236 and 237. | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:11:45 | 6 | |
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Mef2A-shRNA Resource Report Resource Website |
RRID:Addgene_32899 | Mef2a | Rattus norvegicus | Ampicillin | PMID:16484497 | Can be used in conjunction with pcDNA3-rMEF2A (Addgene #32901) which is mutated so as to not be targeted by this shRNA. | Backbone Marker:OligoEngine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin | 2026-08-01 01:11:46 | 0 | |
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pEGFP-rNFM Resource Report Resource Website 1+ mentions |
RRID:Addgene_32907 | EGFP-rat NFM fusion | Rattus norvegicus | Kanamycin | PMID:10707083 | Plasmid first described in Wang et al. (2000) "Rapid movement of axonal neurofilaments interrupted by prolonged pauses" Nature Cell Biology 2:137-141. | Backbone Marker:Clontech; Backbone Size:4134; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-01 01:11:46 | 1 | |
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pSG5-FLAG-CaMKKbeta with CaMKKalpha RP domain Resource Report Resource Website |
RRID:Addgene_33332 | Calcium/Calmodulin dependent protein kinase kinase beta | Rattus norvegicus | Ampicillin | PMID:21807092 | The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations. | Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | CaMKKbeta RP domain (203-225) replaced with CaMKKalpha (167-189) RP domain; S3V, G96R, and P108L | 2026-08-01 01:11:51 | 0 |
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pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain Resource Report Resource Website |
RRID:Addgene_33331 | Calcium/Calmodulin dependent protein kinase kinase alpha | Rattus norvegicus | Ampicillin | PMID:21807092 | Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | CaMKKalpha RP domain (167-189) was replaced with CaMKKbeta RP domain (203-225) using PCR based technique | 2026-08-01 01:11:51 | 0 | |
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pSG5-HA-CaMKKbeta rat FL WT Resource Report Resource Website |
RRID:Addgene_33321 | Calcium/Calmodulin dependent protein kinase kinase beta | Rattus norvegicus | Ampicillin | PMID:21669867 | The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations. | Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S3V, G96R, and P108L | 2026-08-01 01:11:51 | 0 |
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pSG5-FLAG-CaMKKbeta rat 1-460 D311A Resource Report Resource Website |
RRID:Addgene_33325 | Calcium/Calmodulin dependent protein kinase kinase beta | Rattus norvegicus | Ampicillin | PMID:21807092 | The truncation was made by placing a stop codon at 460, but sequence was not actually removed. The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations. | Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | truncated at 460, D311A (S3V, G96R, and P108L also present) | 2026-08-01 01:11:51 | 0 |
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pSG5-FLAG-CaMKKalpha RP deletion Resource Report Resource Website |
RRID:Addgene_33328 | Calcium/Calmodulin dependent protein kinase kinase alpha | Rattus norvegicus | Ampicillin | PMID:21807092 | Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Deleted RP domain (amino acids 167-189 missing) | 2026-08-01 01:11:51 | 0 | |
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pSG5-FLAG-CaMKKbeta rat S572A Resource Report Resource Website |
RRID:Addgene_33319 | Calcium/Calmodulin dependent protein kinase kinase beta | Rattus norvegicus | Ampicillin | PMID:21669867 | Please note that, according to NCBI numbering, the mutation is actually S571A. | Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S571A | 2026-08-01 01:11:51 | 0 |
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sq5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_24499 | Gs alpha | Rattus norvegicus | Ampicillin and Tetracycline | PMID:8863834 | This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. | Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). | 2026-08-01 01:10:40 | 2 |
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CMV::SypHy A4 Resource Report Resource Website 10+ mentions |
RRID:Addgene_24478 | SypHy | Rattus norvegicus | Kanamycin | PMID:16982422 | The depositing lab notes that the Xba1 and Bcl1 unique restriction digestion sites could be methylated and blocked if the plasmid is grown or maintained in dam+ bacteria. Also note that in the Addgene-generated map below, the feature listed as "GFP(12-708)" is actually pH sensitive pHluorin. There is a mutation S446T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP. | Backbone Marker:Clontech; Backbone Size:3952; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-01 01:10:34 | 16 | |
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pcDNA3-PKCZeta KR HA Tagged Resource Report Resource Website |
RRID:Addgene_24608 | PKCZeta | Rattus norvegicus | Ampicillin | PMID:14657501 | Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K281R mutation in the ATP binding domain causing a kinase dead variant. | 2026-08-01 01:10:40 | 0 | |
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pCIS-krPAM2 Resource Report Resource Website |
RRID:Addgene_25457 | PAM2 | Rattus norvegicus | Ampicillin | PMID:1577852 | Backbone Size:0; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Exon A deleted (exon A extends from nt 1475 to nt 1789 and corresponds to aa393-497). | 2026-08-01 01:10:45 | 0 | |
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pEAK10-His-Myc-Kal9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_25441 | Kal9 | Rattus norvegicus | Ampicillin | PMID:10777487 | There are 2 mutations in the sequence, T46S and N62Y. These sequencing errors are the result of mistakes in initial submission to GenBank by the depositing lab. The sequence should be correct. | Backbone Size:6000; Vector Backbone:pEAK-His-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:45 | 3 | |
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pEGFP-N1-VAPA(1-242) K87D/M89D Resource Report Resource Website 1+ mentions |
RRID:Addgene_18875 | VAP-A | Rattus norvegicus | Kanamycin | PMID:16004875 | Backbone Marker:Clontech; Backbone Size:5000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | double point mutant K87D / M89D | 2026-08-01 01:09:43 | 1 | |
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pcDNA3-rBcan Resource Report Resource Website |
RRID:Addgene_18958 | Brevican | Rattus norvegicus | Ampicillin | PMID:18398576 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:09:48 | 0 | ||
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pcDNA4-rB90 Resource Report Resource Website |
RRID:Addgene_18959 | Brevican C-terminus | Rattus norvegicus | Ampicillin | PMID:18398576 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Rat brevican C-terminus: Signal peptide aa 1-22 between Hind III and EcoRI, followed by aa 396-883 between Eco RI and NotI (EcoRI site was introduced and created two extra aa) | 2026-08-01 01:09:44 | 0 | |
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pCAGGS-PSD95-GCaMP2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18931 | PSD95-GCaMP2 | Rattus norvegicus | Ampicillin | PMID:18350138 | Synthetic construct fused with rat PSD95. | Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:09:43 | 1 |
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