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Species: Synthetic
Genetic Insert: Cas9H840A
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBS(-); Vector Types:Plant Expression, Prime editing destination vector for transient expression in dicot plant protoplast; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34713247
Comments: This vector was modified from pPPED, which is made by Dr. Ning Zhang from Dr. Greg Martin lab.
reference:
1. Targeted genome modifications in soybean with CRISPR/Cas9.
Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA.
BMC Biotechnology. 2015;15:16.
Please visit https://www.biorxiv.org/content/10.1101/2020.07.16.206276v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162469 Copy
Species: phage phiC31
Genetic Insert: phiC31 attB site
Vector Backbone Description: Backbone Size:4670; Vector Backbone:pDEST; Vector Types:Drosophila transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector with phiC31 attB site, for Drosophila transformation into attP landing sites.
Proper citation: RRID:Addgene_30325 Copy
Vector Backbone Description: Backbone Size:9205; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are minor discrepancies between Addgene's quality control sequence and the reference sequence provided by the depositing lab. These discrepancies should not affect plasmid function.
Proper citation: RRID:Addgene_19732 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19680 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19671 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R2-R1)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19679 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6938; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19703 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9637; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20205919
Comments: Two mutations are present in the LEU2 sequence: D52E and V69A
Proper citation: RRID:Addgene_20161 Copy
Species: cloning vector
Genetic Insert: pWS-TK3
Vector Backbone Description: Backbone Size:6379; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18546598
Comments: Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.
Proper citation: RRID:Addgene_20349 Copy
Species: cloning vector
Genetic Insert: pWS-TK6
Vector Backbone Description: Backbone Size:6355; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18546598
Comments: Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.
Proper citation: RRID:Addgene_20350 Copy
Species: bacteria
Genetic Insert: aphA::cat
Vector Backbone Description: Backbone Marker:Skorupski, K., Taylor, R.K.; Backbone Size:4400; Vector Backbone:pKAS32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16750581
Comments: Contains cat which confers resistance to chloramphenicol.
Proper citation: RRID:Addgene_20870 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20960 Copy
Species: Chloramphenicol resistance gene (CmR) - ccdB gene
Genetic Insert: Chloramphenicol resistance gene (CmR) - ccdB gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9400; Vector Backbone:pLenti6/UbC/V5-Dest; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: V5 tag deleted
Proper citation: RRID:Addgene_24179 Copy
Vector Backbone Description: Backbone Marker:Soriano Lab (Addgene Plasmid# 21714); Backbone Size:9846; Vector Backbone:pRosa26-1; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21375737
Comments: Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette.
Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418
Proper citation: RRID:Addgene_24418 Copy
Species: reporter gene Venus
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10474; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24568 Copy
Species: reporter gene eGFP
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10444; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24566 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA.
pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
Proper citation: RRID:Addgene_24588 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK.
Proper citation: RRID:Addgene_18668 Copy
Genetic Insert: Gateway cassette A
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_18656 Copy
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.
Proper citation: RRID:Addgene_18700 Copy
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