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Species: Rattus norvegicus
Genetic Insert: rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase)
Vector Backbone Description: Backbone Marker:Cornelia Gorman; Backbone Size:5400; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12369828
Proper citation: RRID:Addgene_145804 Copy
Species: Rattus norvegicus
Genetic Insert: rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase)
Vector Backbone Description: Backbone Marker:Cornelia Gorman; Backbone Size:5000; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7893699
Proper citation: RRID:Addgene_145802 Copy
Species: Rattus norvegicus
Genetic Insert: beta arrestin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Proper citation: RRID:Addgene_14687 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10097102
Proper citation: RRID:Addgene_14685 Copy
Species: Rattus norvegicus
Genetic Insert: beta arrestin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Proper citation: RRID:Addgene_14693 Copy
Species: Rattus norvegicus
Genetic Insert: myogenin
Vector Backbone Description: Backbone Size:5300; Vector Backbone:EMSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10733231
Proper citation: RRID:Addgene_14712 Copy
Species: Rattus norvegicus
Genetic Insert: MRF4
Vector Backbone Description: Backbone Size:5300; Vector Backbone:EMSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10733231
Proper citation: RRID:Addgene_14713 Copy
Species: Rattus norvegicus
Genetic Insert: TGF beta type I receptor
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8662796
Comments: See Author's map for more information.
A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.
Proper citation: RRID:Addgene_14831 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin2D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:PCDNA4/TO/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12737809
Proper citation: RRID:Addgene_14818 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:PCDNA4/TO/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12737809
Proper citation: RRID:Addgene_14817 Copy
Species: Rattus norvegicus
Genetic Insert: DAG reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKCßII (aa 37–152) from KpnI to XhoI and substitution for the PH domain in CYPHR.
Proper citation: RRID:Addgene_14865 Copy
Species: Rattus norvegicus
Genetic Insert: DAG binding domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: YFP-tagged DAG binding domain (YFP-DBD) was first constructed by fusion of monomeric YFP to the 5'-end of the C1b domain of PKCbeta. This construct was improved for DAG binding by introducing the Y123W point mutation via QuikChange (Stratagene).
Proper citation: RRID:Addgene_14874 Copy
Species: Rattus norvegicus
Genetic Insert: Glp1-R
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1326760
Proper citation: RRID:Addgene_14944 Copy
Species: Rattus norvegicus
Genetic Insert: ERK1
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15006 Copy
Species: Rattus norvegicus
Genetic Insert: p130cas
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11314030
Proper citation: RRID:Addgene_15001 Copy
Species: Rattus norvegicus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Gary Nolan, Stanford; Backbone Size:0; Vector Backbone:pBABE-MN-IRES-E-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839357
Comments: An EcoRI-AhaIII fragment containing the entire Hes1 coding region was blunted and subcloned in the blunted BamHI site of the pGFP virus vector.
Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.
Proper citation: RRID:Addgene_15133 Copy
Species: Rattus norvegicus
Genetic Insert: GSK-3 beta R85
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pXT7-myc; Vector Types:Xenopus RNA expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7667318
Comments: See author's map for details.
Proper citation: RRID:Addgene_15493 Copy
Species: Rattus norvegicus
Genetic Insert: GSK-3 beta
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pXT7-myc; Vector Types:Xenopus RNA expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7667318
Comments: See author's map for details. 1.4 kb SmaI-HincII rat GSK3beta PCR fragment was cloned from BSSK into SmaI site of pJ3m vector. SalI-BglII digested insert was recloned into XhoI and BglII sites of pXT7; later 3' end was modified to replace SacI, EcoRI, ClaI, and KpnI with XhoI and HindIII.
Proper citation: RRID:Addgene_15492 Copy
Species: Rattus norvegicus
Genetic Insert: p70 S6K1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:HA-GST-pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17386266
Proper citation: RRID:Addgene_15511 Copy
Species: Rattus norvegicus
Genetic Insert: USP19 (ER isoform)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:pcDNA 3.1/Myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19015242
Proper citation: RRID:Addgene_155237 Copy
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