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Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5088; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The plasmid is fully sequenced in the coding sequence regions (fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
In addition, the published CaMKII promoter contains 10 basepair differences vs. the wild-type, and that persists here.
Proper citation: RRID:Addgene_64545 Copy
Species: Synthetic
Genetic Insert: 12x PP7 stem-loops
Vector Backbone Description: Vector Backbone:pSF4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64544 Copy
Species: Synthetic
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TACT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X1-STOP
Proper citation: RRID:Addgene_64646 Copy
Species: Synthetic
Genetic Insert: SaCas9-2A-GFP
Vector Backbone Description: Backbone Size:4345; Vector Backbone:pCAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64709 Copy
Species: Synthetic
Genetic Insert: Streptococcus pyogenes guide RNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3979; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23561441
Proper citation: RRID:Addgene_64711 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64759 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64758 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20018668
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64755 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_64754 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64757 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64751 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64762 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64763 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64760 Copy
Species: Synthetic
Genetic Insert: pnGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5968; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24059533
Comments: Please cite Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.
Proper citation: RRID:Addgene_64913 Copy
Species: Synthetic
Genetic Insert: hsGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5968; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25141269
Comments: Please cite Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. Please note that you will also need a pMAH plasmid for mammalian expression. pMAH-POLY (Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.) is also deposited to Addgene, which can be used to replace pMAH-AzF for mammalian applications.
Proper citation: RRID:Addgene_64912 Copy
Species: Synthetic
Genetic Insert: hsGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3954; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25141269
Comments: Please cite: Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. For E. coli expression, you will also need pEVOL-pAzF (Addgene # 31186): https://www.addgene.org/31186/
Proper citation: RRID:Addgene_64911 Copy
Species: Synthetic
Genetic Insert: SEP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24403083
Comments: Note that the coding sequence for SEP is lacking a start codon.
Proper citation: RRID:Addgene_64944 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65141 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65144 Copy
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