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Species: Rattus norvegicus
Genetic Insert: Constitutively active Rheb (S16H)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20062052
Comments: The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32520 Copy
Species: Rattus norvegicus
Genetic Insert: μ2 subunit of clathrin adaptor complex AP-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10228163
Comments: NB: The HA tag (YPYDVPDYALE) was inserted within the protein between residues 236 and 237.
Proper citation: RRID:Addgene_32754 Copy
Species: Rattus norvegicus
Genetic Insert: μ2 subunit of clathrin adaptor complex AP-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10228163
Comments: NB: The HA tag (YPYDVPDYALE) was inserted within the protein, between residues 236 and 237.
Proper citation: RRID:Addgene_32752 Copy
Species: Rattus norvegicus
Genetic Insert: Mef2a
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16484497
Comments: Can be used in conjunction with pcDNA3-rMEF2A (Addgene #32901) which is mutated so as to not be targeted by this shRNA.
Proper citation: RRID:Addgene_32899 Copy
Species: Rattus norvegicus
Genetic Insert: EGFP-rat NFM fusion
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4134; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10707083
Comments: Plasmid first described in Wang et al. (2000) "Rapid movement of axonal neurofilaments interrupted by prolonged pauses" Nature Cell Biology 2:137-141.
Proper citation: RRID:Addgene_32907 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33332 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase alpha
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33331 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33321 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The truncation was made by placing a stop codon at 460, but sequence was not actually removed.
The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33325 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase alpha
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33328 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: Please note that, according to NCBI numbering, the mutation is actually S571A.
Proper citation: RRID:Addgene_33319 Copy
Species: Rattus norvegicus
Genetic Insert: Gs alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8863834
Comments: This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation.
Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24499 Copy
Species: Rattus norvegicus
Genetic Insert: SypHy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3952; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16982422
Comments: The depositing lab notes that the Xba1 and Bcl1 unique restriction digestion sites could be methylated and blocked if the plasmid is grown or maintained in dam+ bacteria.
Also note that in the Addgene-generated map below, the feature listed as "GFP(12-708)" is actually pH sensitive pHluorin.
There is a mutation S446T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.
Proper citation: RRID:Addgene_24478 Copy
Species: Rattus norvegicus
Genetic Insert: PKCZeta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657501
Proper citation: RRID:Addgene_24608 Copy
Species: Rattus norvegicus
Genetic Insert: PAM2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1577852
Proper citation: RRID:Addgene_25457 Copy
Species: Rattus norvegicus
Genetic Insert: Kal9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEAK-His-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777487
Comments: There are 2 mutations in the sequence, T46S and N62Y. These sequencing errors are the result of mistakes in initial submission to GenBank by the depositing lab. The sequence should be correct.
Proper citation: RRID:Addgene_25441 Copy
Species: Rattus norvegicus
Genetic Insert: VAP-A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16004875
Proper citation: RRID:Addgene_18875 Copy
Species: Rattus norvegicus
Genetic Insert: Brevican
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18398576
Proper citation: RRID:Addgene_18958 Copy
Species: Rattus norvegicus
Genetic Insert: Brevican C-terminus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18398576
Proper citation: RRID:Addgene_18959 Copy
Species: Rattus norvegicus
Genetic Insert: PSD95-GCaMP2
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18350138
Comments: Synthetic construct fused with rat PSD95.
Proper citation: RRID:Addgene_18931 Copy
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