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Plasmids are provided by Addgene and DGRC.

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On page 12 showing 221 ~ 240 out of 443,797 results
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  • RRID:Addgene_175594

http://www.addgene.org/175594

Species: Drosophila melanogaster
Genetic Insert: Mesh1
Vector Backbone Description: Vector Backbone:pBbA5a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34990598

Proper citation: RRID:Addgene_175594 Copy   


  • RRID:Addgene_175591

    This resource has 1+ mentions.

http://www.addgene.org/175591

Species: Drosophila melanogaster
Genetic Insert: Mesh1
Vector Backbone Description: Vector Backbone:pBbA5a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34990598

Proper citation: RRID:Addgene_175591 Copy   


  • RRID:Addgene_17566

    This resource has 1+ mentions.

http://www.addgene.org/17566

Species: Drosophila melanogaster
Genetic Insert: mini w+
Vector Backbone Description: Backbone Size:2482; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18621688
Comments: pBDP is a modular minimal cloning vector for specific in vivo genomic targeting of Drosophila melanogaster using PhiC31 integrase. The vector contains mini w+ flanked by AscI sites for easy exchange to any marker of choice as well as a large MCS, and the pMB1 ori from pUC19.

Proper citation: RRID:Addgene_17566 Copy   


  • RRID:Addgene_17584

http://www.addgene.org/17584

Species: Drosophila melanogaster
Genetic Insert: taiman
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11163181

Proper citation: RRID:Addgene_17584 Copy   


  • RRID:Addgene_17585

http://www.addgene.org/17585

Species: Drosophila melanogaster
Genetic Insert: taiman
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11163181
Comments: full length taiman minigene

Proper citation: RRID:Addgene_17585 Copy   


  • RRID:Addgene_17587

http://www.addgene.org/17587

Species: Drosophila melanogaster
Genetic Insert: CG4845
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_17587 Copy   


  • RRID:Addgene_17589

    This resource has 1+ mentions.

http://www.addgene.org/17589

Species: Drosophila melanogaster
Genetic Insert: CG4845
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pMT/V5-His B; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_17589 Copy   


  • RRID:Addgene_176078

    This resource has 1+ mentions.

http://www.addgene.org/176078

Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893

Proper citation: RRID:Addgene_176078 Copy   


  • RRID:Addgene_176079

    This resource has 1+ mentions.

http://www.addgene.org/176079

Species: Drosophila melanogaster
Genetic Insert: L596_g25050.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893

Proper citation: RRID:Addgene_176079 Copy   


  • RRID:Addgene_176082

    This resource has 1+ mentions.

http://www.addgene.org/176082

Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893

Proper citation: RRID:Addgene_176082 Copy   


http://www.addgene.org/176679

Species: Drosophila melanogaster
Genetic Insert: spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
Vector Backbone Description: Vector Backbone:Ac5/V5-HIS; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34819517
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_176679 Copy   


  • RRID:Addgene_170439

http://www.addgene.org/170439

Species: Drosophila melanogaster
Genetic Insert: ssrp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7440; Vector Backbone:pET-DEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242411

Proper citation: RRID:Addgene_170439 Copy   


  • RRID:Addgene_170440

http://www.addgene.org/170440

Species: Drosophila melanogaster
Genetic Insert: dre4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7440; Vector Backbone:pET-DEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242411
Comments: The expressed his-tagged protein, pET-DEST42-dre4 (deleted amino acids 1-20) was efficiently bound to CNBR beads during the affinity purification of the antibodies.

Proper citation: RRID:Addgene_170440 Copy   


  • RRID:Addgene_170516

http://www.addgene.org/170516

Species: Drosophila melanogaster
Genetic Insert: gRNAcore(2.1)-pU6.3
Vector Backbone Description: Backbone Size:2351; Vector Backbone:pDONR221; Vector Types:PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP.; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33782117
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/

Proper citation: RRID:Addgene_170516 Copy   


  • RRID:Addgene_170517

http://www.addgene.org/170517

Species: Drosophila melanogaster
Genetic Insert: gRNAcore(EF)-tRNA(Q)
Vector Backbone Description: Backbone Size:2352; Vector Backbone:pDONR221; Vector Types:PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33782117
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/

Proper citation: RRID:Addgene_170517 Copy   


  • RRID:Addgene_170806

    This resource has 1+ mentions.

http://www.addgene.org/170806

Species: Drosophila melanogaster
Genetic Insert: GFP-Msp300KASH
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid derived from GFP-Msp300KASH fusion provided by Janice Fischer and described in: Yu, J. et al. (2006) The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental biology 289, 336-345, doi:10.1016/j.ydbio.2005.10.027 Plasmid first described in: Ma, J. and Weake, V.M. (2014). Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Journal of Visualized Experiments 85. PMID:24686501.

Proper citation: RRID:Addgene_170806 Copy   


http://www.addgene.org/170807

Species: Drosophila melanogaster
Genetic Insert: 2xFLAG/mCherry-Msp300KASH
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid first described in: Hall H., Medina P., Cooper D.A., Escobedo S.E., Rounds J., Brennan K.J., Vincent C., Miura P., Doerge R. and Weake V.M. (2017). Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence. BMC Genomics. 18(1):894. PMID: 29162050.

Proper citation: RRID:Addgene_170807 Copy   


http://www.addgene.org/17771

Species: Drosophila melanogaster
Genetic Insert: miR-1006 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): miR-1006-psm (gagctcCTATGAATAAGAAATCCATTATAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.

Proper citation: RRID:Addgene_17771 Copy   


http://www.addgene.org/17772

Species: Drosophila melanogaster
Genetic Insert: miR-1003 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): miR-1003-psm (gagctcCTGTGAATATGTAAAAGAGTGAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.

Proper citation: RRID:Addgene_17772 Copy   


http://www.addgene.org/17760

Species: Drosophila melanogaster
Genetic Insert: dme-miR-1006 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.

Proper citation: RRID:Addgene_17760 Copy   



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