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Species: Drosophila melanogaster
Genetic Insert: Mesh1
Vector Backbone Description: Vector Backbone:pBbA5a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34990598
Proper citation: RRID:Addgene_175594 Copy
Species: Drosophila melanogaster
Genetic Insert: Mesh1
Vector Backbone Description: Vector Backbone:pBbA5a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34990598
Proper citation: RRID:Addgene_175591 Copy
Species: Drosophila melanogaster
Genetic Insert: mini w+
Vector Backbone Description: Backbone Size:2482; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18621688
Comments: pBDP is a modular minimal cloning vector for specific in vivo genomic
targeting of Drosophila melanogaster using PhiC31 integrase. The vector
contains mini w+ flanked by AscI sites for easy exchange to any marker of
choice as well as a large MCS, and the pMB1 ori from pUC19.
Proper citation: RRID:Addgene_17566 Copy
Species: Drosophila melanogaster
Genetic Insert: taiman
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11163181
Proper citation: RRID:Addgene_17584 Copy
Species: Drosophila melanogaster
Genetic Insert: taiman
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11163181
Comments: full length taiman minigene
Proper citation: RRID:Addgene_17585 Copy
Species: Drosophila melanogaster
Genetic Insert: CG4845
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17587 Copy
Species: Drosophila melanogaster
Genetic Insert: CG4845
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pMT/V5-His B; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17589 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176078 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g25050.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176079 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176082 Copy
Species: Drosophila melanogaster
Genetic Insert: spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
Vector Backbone Description: Vector Backbone:Ac5/V5-HIS; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34819517
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_176679 Copy
Species: Drosophila melanogaster
Genetic Insert: ssrp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7440; Vector Backbone:pET-DEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242411
Proper citation: RRID:Addgene_170439 Copy
Species: Drosophila melanogaster
Genetic Insert: dre4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7440; Vector Backbone:pET-DEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242411
Comments: The expressed his-tagged protein, pET-DEST42-dre4 (deleted amino acids 1-20) was efficiently bound to CNBR beads during the affinity purification of the antibodies.
Proper citation: RRID:Addgene_170440 Copy
Species: Drosophila melanogaster
Genetic Insert: gRNAcore(2.1)-pU6.3
Vector Backbone Description: Backbone Size:2351; Vector Backbone:pDONR221; Vector Types:PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP.; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33782117
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
Proper citation: RRID:Addgene_170516 Copy
Species: Drosophila melanogaster
Genetic Insert: gRNAcore(EF)-tRNA(Q)
Vector Backbone Description: Backbone Size:2352; Vector Backbone:pDONR221; Vector Types:PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33782117
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
Proper citation: RRID:Addgene_170517 Copy
Species: Drosophila melanogaster
Genetic Insert: GFP-Msp300KASH
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid derived from GFP-Msp300KASH fusion provided by Janice Fischer and described in:
Yu, J. et al. (2006) The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental biology 289, 336-345, doi:10.1016/j.ydbio.2005.10.027
Plasmid first described in:
Ma, J. and Weake, V.M. (2014). Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Journal of Visualized Experiments 85. PMID:24686501.
Proper citation: RRID:Addgene_170806 Copy
Species: Drosophila melanogaster
Genetic Insert: 2xFLAG/mCherry-Msp300KASH
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34022041
Comments: Plasmid first described in:
Hall H., Medina P., Cooper D.A., Escobedo S.E., Rounds J., Brennan K.J., Vincent C., Miura P., Doerge R. and Weake V.M. (2017). Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence. BMC Genomics. 18(1):894. PMID: 29162050.
Proper citation: RRID:Addgene_170807 Copy
Species: Drosophila melanogaster
Genetic Insert: miR-1006 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): miR-1006-psm (gagctcCTATGAATAAGAAATCCATTATAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17771 Copy
Species: Drosophila melanogaster
Genetic Insert: miR-1003 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): miR-1003-psm (gagctcCTGTGAATATGTAAAAGAGTGAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17772 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-miR-1006 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.
Proper citation: RRID:Addgene_17760 Copy
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