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Species:
Genetic Insert: BMPR1BA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/3MDY/
Proper citation: RRID:Addgene_53690 Copy
Species: Human papillomavirus
Genetic Insert: HPV16-E7
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53698 Copy
Species: E. coli
Genetic Insert: αNTD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53731 Copy
Species: Homo sapiens
Genetic Insert: UBE3A 3'UTR
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53695 Copy
Species: Human papillomavirus
Genetic Insert: HPV16-E7
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53696 Copy
Species:
Genetic Insert: ACVR1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/3H9R/
Proper citation: RRID:Addgene_53691 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Gateway; Vector Backbone:pDEST R4-R3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53736 Copy
Species: E.coli
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin and Streptomycin
References:
Comments: FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription.
This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article:
http://www.addgene.org/browse/article/8393/
This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q
antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310
Proper citation: RRID:Addgene_53735 Copy
Species: Human papillomavirus
Genetic Insert: HPV16-E1(partial)
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53699 Copy
Species: Other
Genetic Insert: λCI-β-flap fusion
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53733 Copy
Species: Homo sapiens
Genetic Insert: Wilms tumor 1 associated protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53741 Copy
Species: Other
Genetic Insert: 2x membrane linker
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Use NotI/SP6 for RNA to inject.
Put XbaI/AgeI membrane linker in place of histone2B in XbaI/AgeI cut pCS-H2Bcerulean.
This puts membrane-cerulean between XbaI and SnaBI of pCS.
Proper citation: RRID:Addgene_53749 Copy
Species: Homo sapiens
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53746 Copy
Species: Homo sapiens
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Cut with NotI and use SP6 for sense RNA.
Proper citation: RRID:Addgene_53744 Copy
Species: Homo sapiens
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was created using the QuikChange protocol to perform site directed mutagenesis to make Q69M mutation to convert EYFP of pCS-H2B-EYFP (Addgene plasmid # 53746) into citrine.
Use NotI/SP6 for transcription.
Proper citation: RRID:Addgene_53752 Copy
Species: Homo sapiens
Genetic Insert: Sec8
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53758 Copy
Species: Homo sapiens
Genetic Insert: Sec10
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53759 Copy
Species: Homo sapiens
Genetic Insert: Sec5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53756 Copy
Species: Homo sapiens
Genetic Insert: Sec3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53755 Copy
Species: Homo sapiens
Genetic Insert: Exo84
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53762 Copy
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