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Species: Homo sapiens
Genetic Insert: ACE2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16297991
Comments: promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31112 Copy
Species: Homo sapiens
Genetic Insert: RalBP1 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:6349; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20145037
Comments: shRNA RalBP1 sequence: 5′-GTAGAGAGGACCATGATGT-3'
Proper citation: RRID:Addgene_31115 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17214554
Comments: for Flp mediated site directed integration, tetracycline inducibility
Proper citation: RRID:Addgene_31114 Copy
Species: Homo sapiens
Genetic Insert: beta2-spectrin
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.
The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:
Derivations from published sequence:
2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)
Please refer to the Addgene NGS result for the fully validated sequence.
Proper citation: RRID:Addgene_31070 Copy
Species: Homo sapiens
Genetic Insert: TatSF1 shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19479034
Comments: shRNA TatSF1 Target: GAGTCAGATGACAAGGAAG
NB: This shRNA for TatSF1 and Plasmid #31120 both work very well.
Proper citation: RRID:Addgene_31119 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590126
Proper citation: RRID:Addgene_31065 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590126
Proper citation: RRID:Addgene_31064 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31100 Copy
Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31102 Copy
Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31101 Copy
Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31103 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590126
Proper citation: RRID:Addgene_31062 Copy
Species: Homo sapiens
Genetic Insert: osteopontin promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16297991
Comments: promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31106 Copy
Species: Bos taurus
Genetic Insert: Mature form bovine mitochondrial methionyl-tRNA transformylase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9614118
Proper citation: RRID:Addgene_31174 Copy
Species: Homo sapiens
Genetic Insert: FXYD2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19389850
Proper citation: RRID:Addgene_31056 Copy
Species: Homo sapiens
Genetic Insert: Dynactin-4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21186323
Comments: For the preparation of HA-dynactin-4, GFP was first cut out of pEGFP-N1 vector between HindIII and NotI, and in its place, we introduced a triple HA sequence that was synthesized by PCR and ligated into the vector. To a recombinant ORF sequence of dynactin-4, we added by PCR a 5′ XhoI site, and a HindIII site on its 3′ end. The dynactin-4 construct was then cloned into the modified 3XHA-containing vector.
Proper citation: RRID:Addgene_31058 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20546279
Comments: Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31052 Copy
Species: E. coli
Genetic Insert: E. coli elongation factor Ts
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8765000
Proper citation: RRID:Addgene_31173 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK8
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31043 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK7
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31042 Copy
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