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http://www.addgene.org/176582

Species:
Genetic Insert: pRARE2 plasmid
Vector Backbone Description: Vector Backbone:Strain; Vector Types:; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.07.459228v1 for bioRxiv preprint. Primers for recB deletion verification: Foward - tattttccagtcgtgaaagc Reverse - ttgctgatttcttccatcag

Proper citation: RRID:Addgene_176582 Copy   

•   Source: Addgene

http://www.addgene.org/176581

Species:
Genetic Insert: This is a strain.
Vector Backbone Description: Vector Backbone:Strain; Vector Types:; Bacterial Resistance:None
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.07.459228v1 for bioRxiv preprint. Primers for recBCD deletion verification: Foward - ttgatttactgcccgagagc Reverse - gtcaaccgaatgcagacatc

Proper citation: RRID:Addgene_176581 Copy   

•   Source: Addgene

http://www.addgene.org/176658

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Cloning of sgRNA targeting sequence into BbsI cassette. Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_176658 Copy   

•   Source: Addgene

http://www.addgene.org/176651

Species: Mus musculus
Genetic Insert: mVenus-p27K-
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7082; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_176651 Copy   

•   Source: Addgene

http://www.addgene.org/176653

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Cloning of sgRNA targeting sequence into BbsI cassette. Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_176653 Copy   

•   Source: Addgene

http://www.addgene.org/176656

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Cloning of sgRNA targeting sequence into BbsI cassette. Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_176656 Copy   

•   Source: Addgene

http://www.addgene.org/176655

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Cloning of sgRNA targeting sequence into BbsI cassette. Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_176655 Copy   

•   Source: Addgene

http://www.addgene.org/17655

Species:
Genetic Insert: lac I-SceI tet
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 256 lac operator repeats (XhoI fragment) and the 96 tet operator repeats (XhoI –SacII fragment) from the p16PCbeta plasmid (gift from D. Spector) were cloned into pBluescript. Between the two fragments a single 18nt I-SceI site was inserted (SalI). lac operator repeat: tggaattgtgagcggataacaatt tet operator repeat: tccctatcagtgatagaga This plasmid is not stable in bacteria. Every bacterial stock made will contain correct and incorrect bacteria and when bacteria are grown, you will get colonies which contain the correct plasmid and others which contain recombinant versions. If you check multiple colonies (10-20) in the samples after streaking out a stab, you should find correct ones. The recipient will need to grow the bacteria, isolate individual colonies check them, and make a prep from the correct one. We recommend processing the stab and screening colonies immediately upon receipt.

Proper citation: RRID:Addgene_17655 Copy   

•   Source: Addgene

http://www.addgene.org/17656

Species: Homo sapiens
Genetic Insert: UBF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_17656 Copy   

•   Source: Addgene

http://www.addgene.org/17658

Species: Homo sapiens
Genetic Insert: RPA40
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_17658 Copy   

•   Source: Addgene

http://www.addgene.org/176649

Species: Synthetic
Genetic Insert: N-terminus of PE2
Vector Backbone Description: Backbone Size:3544; Vector Backbone:px601; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_176649 Copy   

•   Source: Addgene

http://www.addgene.org/17660

Species: Mus musculus
Genetic Insert: RPA194
Vector Backbone Description: Backbone Size:4730; Vector Backbone:modified pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_17660 Copy   

•   Source: Addgene

http://www.addgene.org/17661

Species: Homo sapiens
Genetic Insert: RRN3/TIF1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note: This plasmid contains a F236L mutation in RRN3 compared to the NCBI reference sequence. It is not known how this mutation affects plasmid function.

Proper citation: RRID:Addgene_17661 Copy   

•   Source: Addgene

http://www.addgene.org/17662

Species: Homo sapiens
Genetic Insert: GFP-lamin A
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: This vector was generated by cloning the NheI–BamHI fragment from pEGFP–wt-lamin A into the BamHI site of the pBABE-puro vector after filling of the ends.

Proper citation: RRID:Addgene_17662 Copy   

•   Source: Addgene

http://www.addgene.org/176643

Species: Synthetic
Genetic Insert: MBP-RGG-LgBit-RGG
Vector Backbone Description: Vector Backbone:pBamUK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_176643 Copy   

•   Source: Addgene

http://www.addgene.org/17663

Species: Homo sapiens
Genetic Insert: GFP-D50 lamin A
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: This vector was generated by cloning the NheI–BamHI fragment from pEGFP–D50 lamin A into the BamHI site of the pBABE-puro vector after filling of the ends. This plasmid exists as a dimer in our stock. Dimerziation often does not impact plasmid function, but may reduce transformation efficiencies. If you need the monomeric version, you could try linearizing, gel extracting, re-ligating, and transforming the plasmid.

Proper citation: RRID:Addgene_17663 Copy   

•   Source: Addgene

http://www.addgene.org/1766

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4888; Vector Backbone:pBABE-zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. SspI site in Amp gene. If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.

Proper citation: RRID:Addgene_1766 Copy   

•   Source: Addgene

http://www.addgene.org/17664

Species: Mus musculus
Genetic Insert: Cx43 CT
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:3800; Vector Backbone:p3XFLAG-CMV-7.1?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_17664 Copy   

•   Source: Addgene

http://www.addgene.org/17665

Species: Mus musculus
Genetic Insert: Cx43 3'UTR
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: 3'UTR of Mus musculus Gja1 (Cx43). For in situ riboprobe. Anti-Sense: use SP6 and cut with ClaI. Sense: use T7 and cut with HindIII.

Proper citation: RRID:Addgene_17665 Copy   

•   Source: Addgene

http://www.addgene.org/17667

Species: Mus musculus
Genetic Insert: Cx43 promoter
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pPD46.20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_17667 Copy   

•   Source: Addgene