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Species: Synthetic
Genetic Insert: CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Backbone Marker:Addgene 171992; Vector Backbone:PEA1-Nuclease-Puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176528 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Backbone Marker:Addgene 171991; Vector Backbone:PEA1-Puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176527 Copy
Species: Homo sapiens
Genetic Insert: Nurr1
Vector Backbone Description: Backbone Size:11000; Vector Backbone:Piggybac TetO - Blastocidin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176485 Copy
Species: Homo sapiens
Genetic Insert: Lmx1A-T2A-Pitx3
Vector Backbone Description: Backbone Size:6585; Vector Backbone:Piggybac TetO - puromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176484 Copy
Species: Mus musculus
Genetic Insert: mouse Wnt1 cDNA
Vector Backbone Description: Backbone Size:8393; Vector Backbone:TetO-FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176486 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.
Proper citation: RRID:Addgene_1765 Copy
Species: Mus musculus
Genetic Insert: trim46
Vector Backbone Description: Vector Backbone:?pGW1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176402 Copy
Species: Homo sapiens
Genetic Insert: En1-t2a-FoxA2
Vector Backbone Description: Backbone Size:8621; Vector Backbone:Piggybac TetO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176483 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-B.1.618
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176438 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-N501Y
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176439 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-W152C
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176431 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-G142D-E154K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176434 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-E154K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176433 Copy
Species: Homo sapiens
Genetic Insert: Alix (360-702)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17639 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-B.1.351
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176426 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-P.1
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176429 Copy
Species: Homo sapiens
Genetic Insert: NR2E3 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176386 Copy
Species: Homo sapiens
Genetic Insert: ZC3H10 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176385 Copy
Species:
Genetic Insert: SARS-CoV-2-NTD-B.1.1.7
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176424 Copy
Species: Homo sapiens
Genetic Insert: BACH1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5493; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17643 Copy
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