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Species: Mus musculus
Genetic Insert: dynein regulatory complex subunit 7
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176470 Copy
Species: Other
Genetic Insert: 4x SV40 polyA
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript II SK(-); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176506 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176462 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176465 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: EYFP is internally inserted to replace the alternative skipping exon 40 of VPS13D (in-frame)
Proper citation: RRID:Addgene_176464 Copy
Species: Mus musculus
Genetic Insert: dynein regulatory complex subunit 3
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176467 Copy
Species: Homo sapiens
Genetic Insert: EGFP-T2A-TFEB(human; delta30; amino acids 31-476)
Vector Backbone Description: Vector Backbone:pCamR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_176500 Copy
Species: Synthetic
Genetic Insert: BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
Vector Backbone Description: Vector Backbone:piggyBac-EF1alpha; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid name in Yang lab: HGpl268
Proper citation: RRID:Addgene_176461 Copy
Species: Ktedonobacter racemifer
Genetic Insert: non-coding locus components from KraIscB-1 locus
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pCOLADuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176537 Copy
Species: Allochromatium warmingii, Brachypodium distachyon
Genetic Insert: AwaIscB
Vector Backbone Description: Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176539 Copy
Species:
Genetic Insert: puroR-T2A-TetOn3G
Vector Backbone Description: Vector Backbone:pCamR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Addgene QC identified a P93S difference in the PuroR selectable marker that the depositing lab does not believe to affect function.
Proper citation: RRID:Addgene_176496 Copy
Species: Homo sapiens
Genetic Insert: HP1 beta
Vector Backbone Description: Backbone Marker:Zentgraf Lab; Backbone Size:0; Vector Backbone:pBCHGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See author's map for more information.
Proper citation: RRID:Addgene_17651 Copy
Species: Homo sapiens
Genetic Insert: RBBP7
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
References:
Comments: SGC link: http://www.thesgc.org/structures/7M3X/.
Proper citation: RRID:Addgene_176411 Copy
Species: Homo sapiens
Genetic Insert: EGFP-T2A-TFEB(human; full-length; S3E mutation)-
Vector Backbone Description: Vector Backbone:pCamR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_176499 Copy
Species: Homo sapiens
Genetic Insert: HP1 alpha
Vector Backbone Description: Backbone Marker:Zentgraf Lab; Backbone Size:0; Vector Backbone:pBCHGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See author's map for more information.
Proper citation: RRID:Addgene_17652 Copy
Species: Homo sapiens
Genetic Insert: D50 lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_17653 Copy
Species: E. coli, Brachypodium distachyon, Delaware Bay metagenome
Genetic Insert: CRISPR-associated IscB (Delaware Bay metagenome)
Vector Backbone Description: Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176534 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mitochondrial FAD-linked sulfhydryl oxidase
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176405 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:VectorBuilder; Backbone Size:598; Vector Backbone:LivePAR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176526 Copy
Species: Other
Genetic Insert: FAD-linked sulfhydryl oxidase ERV2 from Fol
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176404 Copy
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